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147 result(s) for "Guo, Hong-Peng"
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Surgical treatment outcomes of acetabular posterior wall and posterior column fractures using 3D printing technology and individualized custom-made metal plates: a retrospective study
Background Fractures involving the posterior acetabulum with its rich vascular and neural supply present challenges in trauma orthopedics. This study evaluates the effectiveness of 3D printing technology with the use of custom-made metal plates in the treatment of posterior wall and column acetabular fractures. Methods A retrospective analysis included 31 patients undergoing surgical fixation for posterior wall and column fractures of the acetabulum (16 in the 3D printing group, utilizing 3D printing for a 1:1 pelvic model and custom-made plates based on preoperative simulation; 15 in the traditional group, using conventional methods). Surgical and instrument operation times, intraoperative fluoroscopy frequency, intraoperative blood loss, fracture reduction quality, fracture healing time, preoperative and 12-month postoperative pain scores (Numeric Rating Scale, NRS), hip joint function at 6 and 12 months (Harris scores), and complications were compared. Results The surgical and instrument operation times were significantly shorter in the 3D printing group ( p  < 0.001). The 3D printing group exhibited significantly lower intraoperative fluoroscopy frequency and blood loss ( p  = 0.001 and p  < 0.001, respectively). No significant differences were observed between the two groups in terms of fracture reduction quality, fracture healing time, preoperative pain scores (NRS scores), and 6-month hip joint function (Harris scores) ( p  > 0.05). However, at 12 months, hip joint function and pain scores were significantly better in the 3D printing group ( p  < 0.05). Although the incidence of complications was lower in the 3D printing group (18.8% vs. 33.3%), the difference did not reach statistical significance ( p  = 0.433). Conclusion Combining 3D printing with individualized custom-made metal plates for acetabular posterior wall and column fractures reduces surgery and instrument time, minimizes intraoperative procedures and blood loss, enhancing long-term hip joint function recovery. Clinical Trial Registration 12/04/2023;Trial Registration No. ChiCTR2300070438; http://www.chictr.org.cn .
Oral administration of Anabaena-expressed VP28 for both drug and food against white spot syndrome virus in shrimp
White spot syndrome virus (WSSV) is one of the most prevalent and lethal viruses for shrimp, and oral administration of VP28 is a promising approach to protect shrimp against WSSV. Although seven heterologous expression systems have been successful since 2004, no one has been commercialized in shrimp industry. This paper presented the idea of “both drug and feed from the same feedstock” to develop oral vaccine against WSSV. Filamentous, heterocystous cyanobacterium, Anabaena sp. PCC 7120, was used to express major envelope protein, VP28, for feeding post larvae of shrimp. The expression of vp28 gene in transgenic mutant was analyzed by Western blotting and real-time quantitative polymerase chain reaction (RT-qPCR). For vaccination, Litopenaeus vannamei was orally administrated by feeding the wild type and mutants of Anabaena cells for 10 days, and was challenged with WSSV during 10 days post vaccination. The post larvae were fed with the mutant harboring vp28 (10 μg shrimp⁻¹). The post larvae were divided five groups and their survivals were as follows: (1) negative control (neither vaccination nor challenge), 100 %; (2) positive control (challenge and no vaccination), 0 %; (3) feeding the wild type, 15.5 %; (4) feeding blank, 20.6 %; and (5) feeding the mutant harboring vp28, 68.0 %. Moreover, the post larvae fed the mutant harboring vp28 grew better than no feed. Also, the photosynthetic features indicated the transgenic mutant was easier to cultivate. These data show that transgenic mutant harboring vp28 was favorable not only to use both for drug and feed but also to cultivate in scale.
The Study of Prediction of Agricultural Source Pollution Emissions of Ammonia Nitrogen (AN) and Chemical Oxygen Demand (COD) Based on Bionic Neural Network Algorithm
Nearly 40% of Chinese water pollution comes from agricultural sources of pollution, and the annual emissions are difference. If we want to control pollution emissions effectively, we need to accurately predict the amount of agricultural emissions of Ammonia Nitrogen (AN) and Chemical Oxygen Demand (COD). Due to the complex mechanism of the agricultural non-point source pollution, its emissions are very difficult to measure. Currently, the Bionics Research is in a stage of rapid development, and it continues to expand into many new areas of research. So the comprehensive study of Bionics and pollutant control study will be a good choice. This research used bionic BP(Back Propagation) neural network algorithm, and used pollution census data from 2002 to 2007 and established neural network model with neural network algorithm. And we predicted the agricultural sources of emissions of AN and COD with the data from 2008 to 2010. Finally we compared the predicted value and the actual value. Research results showed that, with using the bionic BP neural network, agricultural sources emissions of AN and COD are evaluated actually and the results indicate that the average error is under 5.0%. Research results proved that the model is effective. The neural network is a scientific predict method for the agricultural sources emissions of AN and COD. It can be widely used in the prediction of agricultural sources emissions of AN and COD.
Deposition of TiN/TiAlN multilayers by plasma-activated EB-PVD:tailored microstructure by jumping beam technology
Plasma-activated electron beam-physical vapor deposition(EB-PVD)was used for depositing nitride multilayer coatings in this work.Different from the conventional coating methods,the multilayers were obtained by manipulating electron beam(EB)to jump between two different evaporation sources alternately with variable frequencies(jumping beam technology).The plasma activation was generated by a hollow cathode plasma unit.The deposition process was demonstrated by means of tailoring TiN/TiAlN multilayers with different modulation periods(M1:26.5 nm,M2:80.0 nm,M3:6.0 nm,M4:4.0 nm).The microstructure and hardness of the multilayer coatings were comparatively studied with TiN and TiAlN singlelayer coatings.The columnar structure of the coatings(TiN,TiAlN,M1,M2)is replaced by a glassy-like microstructure when the modulation period decreases to less than 10 nm(M3,M4).Simultaneously,superlattice growth occurs.With the decrease of modulation period,both the hardness and the plastic deformation resistance(H~3/E~2,H-hardness and E-elastic modulus)increase.M4coating exhibits the maximum hardness of(49.6±2.7)GPa and the maximum plastic deformation resistance of~0.74 GPa.
Preliminary Study on Time Budget and Foraging Strategy of Wintering Oriental White Stork at Poyang Lake Jiangxi Province China
The time budgets behavior rhythms foraging habitats and food preferences of oriental white stork Ciconia boyciana were studied from December 2013 to March 2014 with instantaneous scan sampling and focus animal sampling methods at Poyang Lake Jiangxi province China. The results showed that the main behavior of oriental white stork were resting (40.073.21%) and foraging (35.442.42%) followed by moving (13.461.01%) and preening (9.050.62%). The patterns of time budgets were similar in early and later wintering periods. Daily minimum temperature showed a significant linear relationship with foraging and resting. Foraging was significantly positively correlated with daily minimum temperature and resting was significantly negatively correlated with daily minimum temperature which indicated that minimum temperature was the key factor affecting stork behavior in winter. Foraging behavior peaked at 7:007:59 and 16:0016:59 hours and resting peaked at 11:0011:59 hour. Oriental white stork foraging habitats varied in different months and gradually shifted from shallow water to grassland. Their average moving rate was 17.860.61 steps/min pecking rate was 8.310.25 and pecking success rate was 1.030.04 pecks/min. In shallow water average pecking rate was significantly positively correlated with moving rate and pecking success rate. In mudflats and grasslands moving rate was significantly negatively correlated with pecking success rate. We argued that oriental white stork use different foraging methods and prey capture techniques in different habitats possibly because food resources in mudflats and grasslands differ from those in shallow water. The oriental white stork mainly preyed on fish in shallow water and it handled large-sized fishes (greater than 15 cm) for 201.0735.31 sec (n=23) and small-sized fishes (less than 15 cm) for 85.22 20.86 sec (n=14).
Cyclic oxidation behavior of Cr-/Si-modified NiAlHf coatings on single-crystal superalloy produced by EB-PVD
The Cr-/Si-modified Ni Al Hf coatings were produced on single-crystal(SC) superalloy N5 by electron beam physical vapor deposition(EB-PVD). The cyclic oxidation behavior of the coatings at 1100 °C was investigated. The microstructures of the oxide scales grown on the coatings were characterized by scanning electron microscope(SEM) with energy-dispersive X-ray spectrum(EDX),electron probe micro-analyzer(EPMA) and X-ray diffraction(XRD). The effects of Cr and Si on the cyclic oxidation behavior of the Ni Al Hf coatings were discussed. The addition of Si to the Ni Al Hf Cr coating not only reduces the oxidation rate but also enhances the oxide scale adherence.Owing to the addition of Si in the coating, the segregation of Cr and Mo beneath the oxide scale is effectively avoided,which contributes to enhancing oxide scale adherence.
Changes of hepatic biochemical parameters and proteomics in broilers with cold-induced ascites
Ascites syndrome is still a problem for chicken industry in various parts of the world. Despite the intensive investigations of this syndrome for many years, its pathogenesis remains unclear. The objective of this study was to analyze the difference in hepatic proteomics between ascites and healthy broilers by two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS). Changes of biochemical parameters of liver and blood were also determined. The results indicated that red blood cell counts (RBC), hematocrit (HCT) and haemoglobin (HGB) of ascites broilers were significantly greater than healthy broilers. Hepatic malondialdehyde (MDA) level of ascites broilers was significantly increased, and the activity of total superoxide dismutase (T-SOD) was significantly decreased. Hepatic lactic acid (LD) level of ascitic broilers were significantly lower than healthy ones, Serum glucose and cholesterol level of ascites broilers were significantly increased, and serum globulin level was significantly decreased in ascites broilers. There was no significant difference in triglyceride (TG) and blood urea nitrogen (BUN) level. The activity of liver hexokinase (HK) and succinodehydrogenase (SDH) in ascites broilers was significantly decreased, and there was no significant difference in the activity of liver pyruvate kinase (PK) and Na+-K+-ATPase. The hepatic proteomics analysis showed that 18 proteins expression difference were identified between ascites and healthy broilers. These proteins were mainly involved in: 1) cytoskeleton; 2) glucose, lipids and amino acid metabolism; 3) cell secretion; 4) cell apoptosis; 5) signal transduction; 6) immune and inflammatory response; and 7) cellular redox homeostasis. Mitochondrial isoform phosphoenolpyruvate carboxykinase (M-PEPCK) mainly participates in gluconeogenesis of chicken liver. In conclusion, liver oxidative damage was significantly aggravated, but antioxidant capacity was decreased in cold-induced ascites broilers. Serum glucose level was significantly increased, with liver M-PEPCK expression higher in ascites broilers, which implied that some potential regulatory reagents may reduce ascites susceptibility and mortality under cold temperature by increasing liver gluconeogenesis level.
Oxidation behaviour of electron beam physical vapour deposition β-NiAIHf coatings at 1100 ℃ in dry and humid atmospheres
NiA1 intermetallic compounds attract increas- ing attention for high-temperature applications due to their combination of excellent properties, especially as metallic protective coatings on superalloys or as bond coats in thermal barrier coatings (TBCs). Oxidation behaviour of the β-NiA1Hf coatings prepared by electron beam physical vapour deposition (EB-PVD) was investigated at 1100 ℃ for short time and 100h in 20% 02+Ar and 15 % H20 + Ar, respectively. The results of mass changes re- veal that the addition of minor reactive element Hf sig- nificantly improves the cyclic oxidation resistance of the 13- NiA1 coatings in both dry and humid atmospheres. During the initial oxidation stage, water vapour retards the phase transformation from 0-A1203 to a-A1203. Moreover, com- pared with the case in dry atmosphere, water vapour sig- nificantly reduces the surface roughness of the oxide scales formed on EB-PVD β-NiA1Hf coatings after 100 h cyclic oxidation, which corresponds with the difference of surface morphologies of the oxide scales.
Clinical outcomes of fertility-sparing treatments in young patients with epithelial ovarian carcinoma
Objective: To assess the clinical outcomes of fertility-sparing treatments in young patients with epithelial ovarian carcinoma (EOC). Methods: A retrospective study of young EOC inpatients (≤40 years old) was performed during January 1994 and December 2010 in eight institutions. Results: Data were analyzed from 94 patients treated with fertility-sparing surgery with a median follow-up time of 58.7 months. As histologic grade increased, overall survival (OS) and disease-free survival (DFS) of patients receiving fertility-sparing surgery declined. Neither staging surgery nor laparoscopy of early stage EOC with conservative surgery had a significant effect on OS or DFS. Normal menstruation recommenced after chemotherapy in 89% of the fertility-sparing group. Seventeen pregnancies among twelve patients were achieved by the end of the follow-ups. Conclusions: Fertility-sparing treatment for patients with EOC Stage I Grade 1 could be cautiously considered for young patients. The surgical procedure and surgical route might not significantly influence the prognosis. Standard chemotherapy is not likely to have an evident impact on ovarian function or fertility in young patients.
Practice of Hot Metal Full Pretreatment and Less-slag Steelmaking in ShouGangJingTang
The hot metal full pretreatment and less-slag steelmaking process constituted a platform to produce clean steel with low cost and rapid pace and also realized a professional production line. The w[P] can be less than 0.030% at the end of De-P converter and 0.006% at the end of De-C converter. The w[N] in steel can be less than 15×10-6 at the stirring station. This production line can make clean steel steadily used for auto sheet or pipeline, for example X65 MS. The De-C slag was left in the converter for the next heat and recycled to De-P converter to reduce lime consumption, which has a great benefit for the cost saving.