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Rotaviruses are a major etiologic agent of gastroenteritis in infants and young children worldwide. Since the latter of the 1990s, G3 human rotaviruses referred to as \"new variant G3\" have emerged and spread in China, being a dominant genotype until 2010, although their genomic evolution has not yet been well investigated. The complete genomes of 33 G3P[8] human rotavirus strains detected in Wuhan, China, from 2000 through 2013 were analyzed. Phylogenetic trees of concatenated sequences of all the RNA segments and individual genes were constructed together with published rotavirus sequences. Genotypes of 11 gene segments of all the 33 strains were assigned to G3-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1, belonging to Wa genogroup. Phylogenetic analysis of the concatenated full genome sequences indicated that all the modern G3P[8] strains were assigned to Cluster 2 containing only one clade of G3P[8] strains in the US detected in the 1970s, which was distinct from Cluster 1 comprising most of old G3P[8] strains. While main lineages of all the 11 gene segments persisted during the study period, different lineages appeared occasionally in RNA segments encoding VP1, VP4, VP6, and NSP1-NSP5, exhibiting various allele constellations. In contrast, only a single lineage was detected for VP7, VP2, and VP3 genes. Remarkable lineage shift was observed for NSP1 gene; lineage A1-2 emerged in 2007 and became dominant in 2008-2009 epidemic season, while lineage A1-1 persisted throughout the study period. Chinese G3P[8] rotavirus strains have evolved since 2000 by intra-genogroup reassortment with co-circulating strains, accumulating more reassorted genes over the years. This is the first large-scale whole genome-based study to assess the long-term evolution of common human rotaviruses (G3P[8]) in an Asian country.

In the present work, attention is paid to the nonlinear vibrations and chaotic dynamic behaviors of rotating graphene platelets reinforced metal foams (GPLRMF) blades operating in a thermal environment. Considering three different distribution patterns of graphene platelets (GPL) and foams, the improved Halpin–Tsai model, mixing rules and Maxwell-Eucken model are applied to obtain the physical parameters of rotating GPLRMF plates. The motion equations of GPLRMF rotating plates are established through higher-order shear deformation theory (HSDT), in which the centrifugal force, Coriolis force and heat conduction are included. Under the cantilever and simply supported boundary conditions, the nonlinear ordinary differential equation (ODE) of the system is obtained by Galerkin method. The amplitude-frequency response, bifurcation curve, and chaotic motion of the rotating GPLRMF blades are analyzed with the aid of the methods of multi-scale and Runge–Kutta. Furthermore, comprehensive investigations into the effects of temperature, presetting angle, GPL distribution mode, foam distribution mode, volume fraction, porosity coefficient, rotational speed, damping coefficient, and excitation force on the nonlinear dynamics of rotating plates are performed through numerical analyses.

Difficult-to-machine material parts with curved surface are widely used in automobile, aviation, and aerospace fields, and the high-speed milling is the preferred processing method. In high-speed milling process of the curved surface parts, the instantaneous cutting amount is continuously changing due to the geometric feature change of the determined curve toolpath. In this way, the actual processing parameters, including cutting depth, cutting speed, and feed per tooth, are also continuously changing along the curve toolpath, which will result in a severe variation of cutting heat that has negative impact on the tool wear and the surface integrity. For TC4 which is a kind of typical difficult-to-machine material, a novel analysis method of cutting heat is proposed by transforming from time-varying variable to constant parameter for the dry milling of TC4 curved surface. As the actual processing parameters have strong correlational relationship with the instantaneous processing parameters, including instantaneous cutting area, maximum effective cutting radius, and maximum undeformed cutting thickness, the correlational relationship between the actual processing parameters and instantaneous processing parameters is established firstly. By means of fine-tuning the allowance for finish machining and regenerating the curve toolpath, the instantaneous processing parameters can be adjusted to be constant, and the transforming from time-varying variable (actual processing parameters, including cutting depth, actual cutting speed, and feed per tooth) to constant parameter (instantaneous processing parameters, including instantaneous cutting area, maximum effective cutting radius, and maximum undeformed cutting thickness) is realized. Finally, a series of milling experiments are carried out based on the regenerated curve toolpath. The results show that the cutting temperature fluctuation in curved surface milling along the regenerated curve toolpath is decreased by at least 75.81% compared with that in traditional curved surface machining, which is closer to the variation tendency of cutting temperature in plane milling. Consequently, the cutting temperature variation can be smoother by maintaining the instantaneous processing parameters constant, which is of great significance to restrain tool wear and improve surface integrity. Furthermore, the achievement provides theoretical guidance for the allowance distribution of finish machining in the dry milling of TC4 curved surface.

Heroin addicts are a high-risk group for hepatitis C virus (HCV) infection and the development of chronic HCV disease. We thus examined whether morphine, the active metabolite of heroin, has the ability to inhibit intrahepatic interferon (IFN)-α expression, facilitating HCV replication in human hepatocytes. Morphine inhibited intrahepatic IFN-α expression, which was associated with an increase in HCV replication in hepatocytes. Moreover, morphine compromised the anti-HCV effect of recombinant IFN-α. Investigation of the mechanism responsible for the morphine action revealed that morphine inhibited expression of IFN regulatory factor 5 in the hepatocytes. In addition, morphine suppressed the expression of p38, an important signaltransducing molecule involved in IFN-α-mediated anti-HCV activity. These findings indicate that morphine plays a cofactor role in facilitating HCV persistence in human hepatocytes.

To study epidemiological features and genetic characteristics of noroviruses in children and adults with acute gastroenteritis, fecal specimens were collected in three hospitals from Jan. 2007 to May 2010 in Wuhan, China. Noroviruses were detected in 25.9 % (286/1103) and 24.6 % (202/822) of the specimens from children and adults, respectively, with genogroup II (GII) being predominant (99.2 %). The most frequent genotype among GII strains was GII.4 (2006b variant) (77.3 %) (72.0 % in children and 87.9 % in adults), followed by GII.3 (15.0 %) and GII.6 (3.4 %). Potential recombinant genotypes (polymerase/capsid) were detected in 51 GII strains (15.9 %), including the most frequent type, GII.12/GII.3 (28 strains), and GII.16/GII.2, detected for the first time in China, which were found in only children. The results indicated that genetically similar noroviruses were circulating among children and adults as a cause of gastroenteritis, except for some recombinant genotypes.

Based on the Veneziano ghost theory of QCD, we predict the cosmological constant Λ, which is related to energy density of cosmological vacuum by Λ = 8 π G 3 ρ Λ . In the Veneziano ghost theory, the vacuum energy density ρΛ is expressed by absolute value of the product of quark vacuum condensate and quark current mass: ρ Λ = 2 N f H m η ' c | m q 〈 0 | ∶ q ¯ q ∶ | 0 〉 | . We calculate the quark local vacuum condensates 〈 0 | ∶ q ¯ q ∶ | 0 〉 by solving Dyson-Schwinger Equations for a fully dressed confining quark propagator Sf( p) with an effective gluon propagator G μ ν a b ( q ) . The quark current mass mq is predicted by use of chiral perturbation theory. Our theoretical result of Λ, with the resulting 〈 0 | ∶ q ¯ q ∶ | 0 〉 = - ( 235 MeV ) 3 and light quark current mass mq m q ≃ 3.29 - 6.15 M e V is in a good agreement with the observable of the Λ ≈ 10 - 52 m - 2 used widely in a great amount of literatures.

Hospital-based surveillance of rotavirus genotypes was conducted in Wuhan, China, between March 2008 and May 2011. The detection rates of group A rotavirus were 24.6% (458/1859) and 12.1% (96/795) in children and adults, respectively, with diarrhea. Among the 554 positive specimens, the most frequent genotype was G3P[8] (57.9%), followed by G1P[8] (29.4%). Compared with previous studies in Wuhan (2000-2008), the relative frequency of G3P[8] has been decreasing year by year, while the predominant genotype G3 shifted to G1 in 2011. In the present study, a rare P[8]b subtype of the VP4 gene (OP354-like P[8]) was identified in nine strains. Full-length sequences of VP7, VP4, VP6 and NSP4 genes of two G9P[8]b strains (RVA/Human-wt/CHN/E1545/2009/G9P[8]b and RVA/Human-wt/CHN/Z1108/2008/G9P[8]b) were determined for phylogenetic analysis. The four genes of these strains were closely related to one another, and the G9-VP7 genes of these strains belonged to lineage III, which contains globally spreading G9 rotaviruses. The full-length sequence of VP4 gene segments of the P[8]b strains in Wuhan clustered with those of P[8]b strains in Vietnam, Russia and Belgium, while they were distinct from those of the OP354 strain from Malawi and Bangladeshi strains. The VP6 and NSP4 genes of two P[8]b strains belonged to the I1 and E1 genotype, respectively, and clustered with those of strains belonging to Wa-like human rotaviruses from various Asian countries. These findings indicate the changing epidemiologic trend of rotavirus genotypes in Wuhan, i.e., the shift of the predominant type from G3 to G1 and the emergence of P[8]b strains genetically related to those distributed in other Asian countries.

Background. Hemorrhagic fever-like illness caused by a novel Bunyavirus, Huaiyangshan virus (HYSV, also known as Severe Fever with Thrombocytopenia virus [SFTSV] and Fever, Thrombocytopenia and Leukopenia Syndrome [FTLS]), has recently been described in China. Methods. Patients with laboratory-confirmed HYSV infection who were admitted to Union Hospital or Zhongnan Hospital between April 2010 and October 2010 were included in this study. Clinical and routine laboratory data were collected and blood, throat swab, urine, or feces were obtained when possible. Viral RNA was quantified by real-time reverse-transcriptase polymerase chain reaction. Blood levels of a range of cytokines, chemokines, and acute phase proteins were assayed. Results. A total of 49 patients with hemorrhagic fever caused by HYSV were included; 8 (16.3%) patients died. A fatal outcome was associated with high viral RNA load in blood at admission, as well as higher serum liver transaminase levels, more pronounced coagulation disturbances (activated partial thromboplastin time, thrombin time), and higher levels of acute phase proteins (phospholipase A, fibrinogen, hepcidin), cytokines (interleukin [IL]-6, IL-10, interferon-γ), and chemokines (IL-8, monocyte chemotactic protein 1, macrophage inflammatory protein lb). The levels of these host parameters correlated with viral RNA levels. Blood viral RNA levels gradually declined over 3-4 weeks after illness onset, accompanied by resolution of symptoms and laboratory abnormalities. Viral RNA was also detectable in throat, urine, and fecal specimens of a substantial proportion of patients, including all fatal cases assayed. Conclusions. Viral replication and host immune responses play an important role in determining the severity and clinical outcome in patients with infection by HYSV.

Rotaviruses are a major etiologic agent of gastroenteritis in infants and young children worldwide. Since the latter of the 1990s, G3 human rotaviruses referred to as \"new variant G3\" have emerged and spread in China, being a dominant genotype until 2010, although their genomic evolution has not yet been well investigated.BACKGROUNDRotaviruses are a major etiologic agent of gastroenteritis in infants and young children worldwide. Since the latter of the 1990s, G3 human rotaviruses referred to as \"new variant G3\" have emerged and spread in China, being a dominant genotype until 2010, although their genomic evolution has not yet been well investigated.The complete genomes of 33 G3P[8] human rotavirus strains detected in Wuhan, China, from 2000 through 2013 were analyzed. Phylogenetic trees of concatenated sequences of all the RNA segments and individual genes were constructed together with published rotavirus sequences.METHODSThe complete genomes of 33 G3P[8] human rotavirus strains detected in Wuhan, China, from 2000 through 2013 were analyzed. Phylogenetic trees of concatenated sequences of all the RNA segments and individual genes were constructed together with published rotavirus sequences.Genotypes of 11 gene segments of all the 33 strains were assigned to G3-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1, belonging to Wa genogroup. Phylogenetic analysis of the concatenated full genome sequences indicated that all the modern G3P[8] strains were assigned to Cluster 2 containing only one clade of G3P[8] strains in the US detected in the 1970s, which was distinct from Cluster 1 comprising most of old G3P[8] strains. While main lineages of all the 11 gene segments persisted during the study period, different lineages appeared occasionally in RNA segments encoding VP1, VP4, VP6, and NSP1-NSP5, exhibiting various allele constellations. In contrast, only a single lineage was detected for VP7, VP2, and VP3 genes. Remarkable lineage shift was observed for NSP1 gene; lineage A1-2 emerged in 2007 and became dominant in 2008-2009 epidemic season, while lineage A1-1 persisted throughout the study period.RESULTSGenotypes of 11 gene segments of all the 33 strains were assigned to G3-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1, belonging to Wa genogroup. Phylogenetic analysis of the concatenated full genome sequences indicated that all the modern G3P[8] strains were assigned to Cluster 2 containing only one clade of G3P[8] strains in the US detected in the 1970s, which was distinct from Cluster 1 comprising most of old G3P[8] strains. While main lineages of all the 11 gene segments persisted during the study period, different lineages appeared occasionally in RNA segments encoding VP1, VP4, VP6, and NSP1-NSP5, exhibiting various allele constellations. In contrast, only a single lineage was detected for VP7, VP2, and VP3 genes. Remarkable lineage shift was observed for NSP1 gene; lineage A1-2 emerged in 2007 and became dominant in 2008-2009 epidemic season, while lineage A1-1 persisted throughout the study period.Chinese G3P[8] rotavirus strains have evolved since 2000 by intra-genogroup reassortment with co-circulating strains, accumulating more reassorted genes over the years. This is the first large-scale whole genome-based study to assess the long-term evolution of common human rotaviruses (G3P[8]) in an Asian country.CONCLUSIONChinese G3P[8] rotavirus strains have evolved since 2000 by intra-genogroup reassortment with co-circulating strains, accumulating more reassorted genes over the years. This is the first large-scale whole genome-based study to assess the long-term evolution of common human rotaviruses (G3P[8]) in an Asian country.