Catalogue Search | MBRL
Are you sure you want to remove the book from the shelf?
{{itemTitle}}
138
result(s) for
"Reuter, Wolfgang"
Sort by:
Structural analysis at 2.2 Å of orthorhombic crystals presents the asymmetry of the allophycocyanin–linker complex, AP⋅LC7.8, from phycobilisomes of Mastigocladus laminosus
An electrophoretically purified allophycocyanin–linker complex, AP⋅L C 7.8 , from phycobilisomes of Mastigocladus laminosus has been crystallized in the orthorhombic space group P2 1 2 1 2 1 . Cryocrystallographic x-ray measurements enabled the structural analysis of the complex at a resolution of 2.2 Å. The asymmetric unit contains two side-to-side associated “trimeric” (αβ) 3 allophycocyanin complexes comprising the linker polypeptide in a defined orientation inside the trimer. The linker representing a protein fold related to the prosegment of procarboxypeptidase A is in contact with only two of the three β-subunits and directly interacts with the corresponding chromophores of these proteins. In addition to a modulation of the chromophores’ spectral properties, the linker polypeptide attracts the αβ-subcomplexes, thereby bringing the β-chromophores closer together. These results will enable interpretations of energy-transfer mechanisms within phycobiliproteins. cyanobacteria linker polypeptides crystallization photosynthesis energy transfer
Journal Article
Circulating Vascular Cell Adhesion Molecules VCAM-1, ICAM-1, and E-Selectin in Dependence on Aging
Background: Elevated levels of circulating cell adhesion molecules (cCAMs) such as vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), and endothelial leukocyte adhesion molecule-1 (E-selectin) are found in subjects with vascular diseases and in subjects with several risk factors for atherosclerosis. However, data evaluating cCAMs and biological age are limited. Objective: The purpose of this study was to assess in subjects with different cardiovascular risk profiles the levels of cVCAM-1, cICAM-1, and cE-selectin in dependence on age. Methods: The following groups of subjects were included in the study: 282 apparently healthy subjects of the average population aged 18–89 years, 77 vegetarians who are characterized by a favourable global cardiovascular risk profile, 94 patients with coronary heart disease, and 181 patients with peripheral arterial occlusive disease. Blood samples were obtained after an overnight fast for measurement of cCAMs, lipoproteins, and other clinical/biochemical parameters. The cCAM levels were determined by the use of monoclonal antibody based enzyme-linked immunosorbent assays. Results: Amongst the cCAMs, cVCAM-1 is uniquely elevated in elderly persons with different risks for atherosclerosis, including subjects of the average population, vegetarians with a favourable risk profile, and patients with both coronary heart disease and peripheral arterial occlusive disease. With respect to cICAM-1, an age-dependent elevation was found in the control subjects included in the study. The cE-selectin levels were not correlated with age. Moreover, no associations of cCAMs with serum lipid and lipoprotein levels were found. Conclusion: The results of the present study indicate that cVCAM-1 is an age-dependent parameter independent of cardiovascular risk.
Journal Article
Social Roots of Self-Consciousness
Selbstbewusstsein, die Fähigkeit, über uns nachzudenken, uns unserer Gefühle oder Gedanken bewusst zu sein, ist zweifellos eine unserer markantesten und wichtigsten kognitiven Fähigkeiten. Worin besteht Selbstbewusstsein jedoch genauer, welche Teilfähigkeiten kommen zum Tragen, wenn wir uns unserer Gedanken und Gefühle bewusst sind? Und wie erwerben Kleinkinder im Zuge ihrer Entwicklung diese Fähigkeit? Beide Frageperspektiven sind eng miteinander verzahnt. Da empirische und begriffliche Fragen rund um das Phänomen des Selbstbewusstseins nur durch einen intensiv geführten Dialog zwischen Psychologie und Philosophie angemessen beantwortet werden können, versammelt dieser Band Beiträge von Vertretern beider Disziplinen. Das besondere Augenmerk der in Englisch verfassten Beiträge des Bandes gilt der Frage, welche Rolle die soziale Einbettung von Kleinkindern im Erwerb von Selbstbewusstsein und den mit ihr verknüpften kognitiven Fähigkeiten spielt. Vor allem die Annahme, dass Kleinkinder nur dank bestimmter Arten von sozialen Interaktionen die Fähigkeit entwickeln, sich von anderen Personen in der Welt zu unterscheiden und eine eigene Perspektive auf sich und die Welt einzunehmen, wird aus verschiedenen Blickwinkeln kritisch geprüft.
eBook
A rapid, quantitative immunofunctional assay for measuring human leptin
We have developed a rapid, sensitive and quantitative in vitro assay for leptin based upon its ability to bind to the soluble extracellular domain of the leptin receptor (sOB-R). Such an assay is theoretically capable of differentiating between physiologically active leptin molecules from those with modified, either enhanced or reduced, binding activity.
A preparation of sOB-R was immobilized to capture leptin from serum samples or standards. Anti-leptin antibodies that had been raised in rabbits were added in a second incubation step to identify leptin molecules bound to sOB-R. Signal detection was performed in a third incubation step by anti-rabbit IgG labeled with peroxidase. The immunofunctional assay (IFA) was clinically validated by the comparison of leptin levels in adolescents (n = 41, age range 9-18 years, BMI range 13.4-33.8 kg/m(2) and adults (n = 80, age range 18-77 years, BMI range 16.4-54.7 kg/m(2) measured using the IFA with data of an in-house RIA performed with the same standards and leptin antibodies.
The functional sensitivity of the IFA was 0.4 ng/ml and comparable to the data of the RIA. Intra- and interassay coefficients of variation were below 12.5 % in both methods. Leptin levels correlated well with the BMI of the subjects studied (r = 0.70 for RIA, r = 0.72 for IFA; p < 0.0001) as well as between IFA (y) and RIA (x) (y = x -1.31 ng/ml; r = 0.97, p < 0.0001). The median of the quotient between IFA and RIA levels was 0.86 (quartile range 0.60-1.10) for all samples.
So far, only at the most minor differences between leptin measurements using the newly developed IFA and those using a conventional RIA have been detected. Additional studies using the IFA method are required to investigate whether or not discrepant results with the IFA will be seen in various states of relative leptin resistance and whether or not such differences are of biological relevance.
Journal Article
SEEbalance®: Managing Sustainability of Products and Processes with the Socio-Eco-Efficiency Analysis by BASF
BASF is co-operating with Karlsruhe University and the Öko-Institut e.V., Germany, in developing a method for measuring social aspects of sustainability with the aim of incorporating them into BASF's existing eco-efficiency analysis. Whereas numerous instruments are used in practice for the ecological assessment of products and processes, a gap still has to be closed by developing social life-cycle assessment procedures. Based on the principles of the BASF eco-efficiency analysis on the one hand, and a product-related specification of the social sustainability dimension on the other hand, a social life-cycle assessment procedure has been developed which is now to be tested and discussed. The new integrated instrument, the so-called SEEbalance®, will be applied at BASF in order to improve the performance of the company's product portfolio and manufacturing processes and to market advantageous products. This paper presents the concept of the new methodology. SEEbalance®is a comparative life-cycle assessment tool that consists of three main aspects: costs, environmental impact and social effects of different product or process alternatives are examined and compared. Socio-eco-efficient solutions combine a relatively good environmental performance with high social benefit and at the same time low costs for the end customer. Social criteria for the evaluation of products and processes are proposed. The developed method for the social life-cycle assessment is based on an industrial sector analysis of statistical data. The final aggregation and presentation of the results is done in an analogous manner to the eco-efficiency analysis.
Journal Article
Structural analysis at 2.2 Å of orthorhombic crystals presents the asymmetry of the allophycocyanin–linker complex, AP⋅L C 7.8 , from phycobilisomes of Mastigocladus laminosus
An electrophoretically purified allophycocyanin–linker complex,
AP⋅L
C
7.8
, from phycobilisomes of
Mastigocladus laminosus
has been crystallized in the
orthorhombic space group P2
1
2
1
2
1
.
Cryocrystallographic x-ray measurements enabled the structural analysis
of the complex at a resolution of 2.2 Å. The asymmetric unit contains
two side-to-side associated “trimeric” (αβ)
3
allophycocyanin complexes comprising the linker polypeptide in a
defined orientation inside the trimer. The linker representing a
protein fold related to the prosegment of procarboxypeptidase A is in
contact with only two of the three β-subunits and directly interacts
with the corresponding chromophores of these proteins. In addition to a
modulation of the chromophores’ spectral properties, the linker
polypeptide attracts the αβ-subcomplexes, thereby bringing the
β-chromophores closer together. These results will enable
interpretations of energy-transfer mechanisms within phycobiliproteins.
Journal Article
Structural analysis at 2.2 Angstrom of orthorhombic crystals presents the asymmetry of the allophycocyanin-linker complex, AP-LC7-8, from phycobilisomes of Mastigocladus laminosus
An electrophoretically purified allophyco-cyanin-linker complex, AP-L7.8C, from phycobilisomes of Mastigocladus laminosus has been crystallized in the orthorhombic space group P212121.
Journal Article
Structural Analysis at 2.2 angstrom of Orthorhombic Crystals Presents the Asymmetry of the Allophycocyanin-Linker Complex,$\\text{AP}· \\text{L}_{\\text{C}}^{7.8}$, from Phycobilisomes of Mastigocladus laminosus
An electrophoretically purified allophycocyanin-linker complex,$\\text{AP}· \\text{L}_{\\text{C}}^{7.8}$, from phycobilisomes of Mastigocladus laminosus has been crystallized in the orthorhombic space group P212121. Cryocrystallographic x-ray measurements enabled the structural analysis of the complex at a resolution of 2.2 angstrom. The asymmetric unit contains two side-to-side associated \"trimeric\" (α β)3allophycocyanin complexes comprising the linker polypeptide in a defined orientation inside the trimer. The linker representing a protein fold related to the prosegment of procarboxypeptidase A is in contact with only two of the three β -subunits and directly interacts with the corresponding chromophores of these proteins. In addition to a modulation of the chromophores' spectral properties, the linker polypeptide attracts the α β -subcomplexes, thereby bringing the β -chromophores closer together. These results will enable interpretations of energy-transfer mechanisms within phycobiliproteins.
Journal Article
Extended High Frequency Audiometry in Pre-school Children
Since adequate and reliable earphones are now available and standards have been established, clinical audiometry can be performed at extended high frequencies (EHFs). In the present study, 47 preschool children (aged 4-7 years) were examined with EHFs (8-16 kHz) using the new Sennheiser HDA 200 earphone. The hearing thresholds correspond to those of other studies; the median thresholds and range increase with increasing frequency. The medians and quartiles were: 10 kHz: 25 (5-35) dB(SPL), 11.2 kHz: 35 (20-40) dB(SPL), 14 kHz 40 (30-50) dB(SPL) and 16 kHz: 50 (40-60) dB(SPL). The hearing thresholds could be more reliably determined in the older children (< 5 years) and were 5 dB better than in the younger ones (< 5 years). Thresholds in the 10-12.5 kHz range correspond to those of adults but are more sensitive by 5-12 dB in the 14-16 kHz range.
Journal Article