Journal Article

Fusobacterium nucleatum Secretes Outer Membrane Vesicles and Promotes Intestinal Inflammation

Ruan, Wenly, Brand, Colleen K., Shi, Zhongcheng, Venable, Susan, Hirschi, Kendal D., Chang-Graham, Alexandra L., Krystofiak, Evan S., Engevik, Kristen A., Liu, Xinli, Versalovic, James, Britton, Robert A., Engevik, Melinda A., Danhof, Heather A., Zhao, Yanling, Engevik, Amy C., Hyser, Joseph M., Spinler, Jennifer K.

2021

Overview

Several studies have identified an increased abundance of Fusobacterium in the intestinal tracts of patients with colon cancer, liver cirrhosis, primary sclerosing cholangitis, gastroesophageal reflux disease, HIV infection, and alcoholism. However, the direct mechanism(s) of action of Fusobacterium on pathophysiological within the gastrointestinal tract is unclear. Multiple studies have implicated microbes in the development of inflammation, but the mechanisms remain unknown. Bacteria in the genus Fusobacterium have been identified in the intestinal mucosa of patients with digestive diseases; thus, we hypothesized that Fusobacterium nucleatum promotes intestinal inflammation. The addition of >50 kDa F. nucleatum conditioned media, which contain outer membrane vesicles (OMVs), to colonic epithelial cells stimulated secretion of the proinflammatory cytokines interleukin-8 (IL-8) and tumor necrosis factor (TNF). In addition, purified F. nucleatum OMVs, but not compounds <50 kDa, stimulated IL-8 and TNF production; which was decreased by pharmacological inhibition of Toll-like receptor 4 (TLR4). These effects were linked to downstream effectors p-ERK, p-CREB, and NF-κB. F. nucleatum >50-kDa compounds also stimulated TNF secretion, p-ERK, p-CREB, and NF-κB activation in human colonoid monolayers. In mice harboring a human microbiota, pretreatment with antibiotics and a single oral gavage of F. nucleatum resulted in inflammation. Compared to mice receiving vehicle control, mice treated with F. nucleatum showed disruption of the colonic architecture, with increased immune cell infiltration and depleted mucus layers. Analysis of mucosal gene expression revealed increased levels of proinflammatory cytokines (KC, TNF, IL-6, IFN-γ, and MCP-1) at day 3 and day 5 in F. nucleatum -treated mice compared to controls. These proinflammatory effects were absent in mice who received F. nucleatum without pretreatment with antibiotics, suggesting that an intact microbiome is protective against F. nucleatum -mediated immune responses. These data provide evidence that F. nucleatum promotes proinflammatory signaling cascade s in the context of a depleted intestinal microbiome. IMPORTANCE Several studies have identified an increased abundance of Fusobacterium in the intestinal tracts of patients with colon cancer, liver cirrhosis, primary sclerosing cholangitis, gastroesophageal reflux disease, HIV infection, and alcoholism. However, the direct mechanism(s) of action of Fusobacterium on pathophysiological within the gastrointestinal tract is unclear. These studies have identified that F. nucleatum subsp. polymorphum releases outer membrane vesicles which activate TLR4 and NF-κB to stimulate proinflammatory signals in vitro . Using mice harboring a human microbiome, we demonstrate that F. nucleatum can promote inflammation, an effect which required antibiotic-mediated alterations in the gut microbiome. Collectively, these results suggest a mechanism by which F. nucleatum may contribute to intestinal inflammation.

• Publisher: American Society for Microbiology
• Subject: Alcoholism / Animals / Antibiotics / Bacterial Outer Membrane - immunology / Cells, Cultured / Cholangitis / Cirrhosis / Colon - cytology / Colon cancer / Colorectal cancer / Culture Media - pharmacology / Cyclic AMP response element-binding protein / Cytokines / Cytokines - analysis / Cytokines - immunology / Digestive system diseases / Disease / Epithelial cells / Epithelial Cells - drug effects / Epithelial Cells - immunology / Experiments / Extracellular Vesicles - immunology / Female / Fusobacterium / Fusobacterium nucleatum / Fusobacterium nucleatum - immunology / Fusobacterium nucleatum - metabolism / Fusobacterium nucleatum - pathogenicity / Gastroesophageal reflux / Gastrointestinal Microbiome / Gastrointestinal tract / Gene expression / HIV / Host-Microbe Biology / HT29 Cells / Human immunodeficiency virus / Humans / Hypotheses / Immune response / Infections / Inflammation / Inflammation - immunology / Inflammation - microbiology / Inflammatory bowel disease / Interleukin 8 / Intestinal microflora / Intestine / Intestines - immunology / Intestines - microbiology / Intestines - pathology / Kinases / Liver cancer / Male / Membrane vesicles / Metabolism / Metastases / Mice / Mice, Inbred C57BL / Microbiomes / Monocyte chemoattractant protein 1 / NF-kappa B - immunology / NF-κB protein / Research Article / Signal Transduction / TLR4 protein / Toll-Like Receptor 4 - immunology / Toll-like receptors / Tumor necrosis factor / Tumor necrosis factor-TNF / γ-Interferon