Journal Article

B lymphocyte-derived acetylcholine limits steady-state and emergency hematopoiesis

Capen, Diane, Masson, Gustavo Santos, Swirski, Filip K, Brown, Dennis, Hulsmans, Maarten, Courties, Gabriel, Nahrendorf, Matthias, Foy, Brody H, Gustafsson, Karin, Schmidt, Stephen P, Higgins, John M, Kokkaliaris, Konstantinos D, Rohde, David, Knudsen, Anders Steen, McAlpine, Cameron S, Naxerova, Kamila, Scadden, David T, Yamazoe, Masahiro, Pulous, Fadi E, Libby, Peter, Zhang, Shuang, Iwamoto, Yoshiko, Schloss, Maximilian J, Wojtkiewicz, Gregory R, Yang, Chongbo, Lee, I-Hsiu, Severe, Nicolas, Frodermann, Vanessa

2022

Overview

Autonomic nerves control organ function through the sympathetic and parasympathetic branches, which have opposite effects. In the bone marrow, sympathetic (adrenergic) nerves promote hematopoiesis; however, how parasympathetic (cholinergic) signals modulate hematopoiesis is unclear. Here, we show that B lymphocytes are an important source of acetylcholine, a neurotransmitter of the parasympathetic nervous system, which reduced hematopoiesis. Single-cell RNA sequencing identified nine clusters of cells that expressed the cholinergic α7 nicotinic receptor (Chrna7) in the bone marrow stem cell niche, including endothelial and mesenchymal stromal cells (MSCs). Deletion of B cell-derived acetylcholine resulted in the differential expression of various genes, including Cxcl12 in leptin receptor+ (LepR+) stromal cells. Pharmacologic inhibition of acetylcholine esterase decreased the systemic supply of inflammatory myeloid cells in mice and humans with cardiovascular disease.Nahrendorf and colleagues show that B cells in the bone marrow are an important source of the neurotransmitter acetylcholine, which limits hematopoiesis through modulating the signals produced by the bone marrow stromal niche during steady-state and emergency hematopoiesis.

• Publisher: Nature Publishing Group
• Subject: Autonomic nervous system / Bone marrow / Cardiovascular diseases / Clonal deletion / CXCL12 protein / Inflammation / Lymphocytes / Lymphocytes B / Mesenchyme / Myeloid cells / Parasympathetic nervous system / Stem cells / Stromal cells