Asset Details
Do you wish to reserve the book?
Systematic discovery of antiphage defense systems in the microbial pangenome
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Systematic discovery of antiphage defense systems in the microbial pangenome
Do you wish to request the book?
Systematic discovery of antiphage defense systems in the microbial pangenome
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Systematic discovery of antiphage defense systems in the microbial pangenome
2018
Overview
To survive the attack of foreign invaders such as viruses and plasmids, bacteria and archaea fight back with immune systems that are usually clustered in “defense islands” in their genomes. Doron et al. took advantage of this property to map microbial defense systems systematically (see the Perspective by Kim). Candidate immune systems were then experimentally validated for their activities. Like well-known defense arsenals such as restriction-modification and CRISPR systems, these additional immune systems now require mechanistic investigation and could potentially be engineered into useful molecular tools in the future. Science , this issue p. eaar4120 ; see also p. 993 Bioinformatics and experimental validation identify nine antiphage and one antiplasmid immune defense systems in microbes. The arms race between bacteria and phages led to the development of sophisticated antiphage defense systems, including CRISPR-Cas and restriction-modification systems. Evidence suggests that known and unknown defense systems are located in “defense islands” in microbial genomes. Here, we comprehensively characterized the bacterial defensive arsenal by examining gene families that are clustered next to known defense genes in prokaryotic genomes. Candidate defense systems were systematically engineered and validated in model bacteria for their antiphage activities. We report nine previously unknown antiphage systems and one antiplasmid system that are widespread in microbes and strongly protect against foreign invaders. These include systems that adopted components of the bacterial flagella and condensin complexes. Our data also suggest a common, ancient ancestry of innate immunity components shared between animals, plants, and bacteria.
Publisher
The American Association for the Advancement of Science
Subject
/ Archaea
/ Bacillus subtilis - genetics
/ Bacillus subtilis - immunology
/ Bacillus subtilis - virology
/ Bacteria
/ Bacteriophages - pathogenicity
/ CRISPR
/ DNA
/ Enzymes
/ Escherichia coli - immunology
/ Flagella
/ Genes
/ Genes, Bacterial - physiology
/ Genomes
/ Immunity
/ Islands
/ Phages
/ Plasmids
/ Viruses
