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Genome-wide analysis of laccase genes in moso bamboo highlights PeLAC10 involved in lignin biosynthesis and in response to abiotic stresses
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Genome-wide analysis of laccase genes in moso bamboo highlights PeLAC10 involved in lignin biosynthesis and in response to abiotic stresses
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Journal Article
Genome-wide analysis of laccase genes in moso bamboo highlights PeLAC10 involved in lignin biosynthesis and in response to abiotic stresses
2020
Overview
Key message
Twenty-three
PeLAC
s have been identified in moso bamboo, overexpression of
PeLAC10
increases the lignin content and confers drought and phenolic acid tolerance in transgenic
Arabidopsis
.
Laccases (LACs) have multifunction involved in the processes of cell elongation, lignification and stress response in plants. However, the function of laccases in bamboo remain unclear. Here, a total of 23 laccase genes (
PeLAC1
–
PeLAC23
) were identified in moso bamboo (
Phyllostachys edulis
). The diverse gene structure and expression pattern of
PeLAC
s suggested that their function should be spatiotemporal and complicated, which was supported by the expression profiles in different tissues of moso bamboo. Eighteen
PeLAC
s were identified as the targets of
ped-
miR397. The putative
ped-
miR397-binding site in the coding region of
PeLAC10
was further confirmed by RLM-5′ RACE, indicating that
PeLAC10
was regulated by
ped-miR397
after transcription. With the increasing shoot height, the expression abundance of
PeLAC10
was up-regulated and reached the maximum in 15 cm shoots, while that of
ped-
miR397 was relative lower and showed the minimum in 15 cm shoots.
PeLAC10
was up-regulated obviously under both ABA (100 μmol L
–1
) and NaCl (400 mmol L
–1
) treatments, and it was down-regulated under the GA
3
(100 μmol L
–1
) treatment. The transgenic
Arabidopsis
plants over-expressing
PeLAC10
became slightly smaller and their petioles were shorter than those of Col-0. However, they had a stronger capacity in resistance to phenolic acids and drought besides higher lignin content in stems. These results indicated that overexpression of
PeLAC10
was helpful to increase the content of lignin in transgenic
Arabidopsis
and improve the adaptability to phenolic acid and drought stresses.
Publisher
Springer Berlin Heidelberg
Subject
/ bamboos
/ Biomedical and Life Sciences
/ drought
/ Droughts
/ Gene Expression Regulation, Plant
/ genes
/ genome-wide association study
/ Hydroxybenzoates - pharmacology
/ laccase
/ lignin
/ petioles
/ Plants, Genetically Modified - genetics
/ shoots
/ stems
/ Stress, Physiological - drug effects
