Journal Article

Multiple biomarker responses (serum biochemistry, oxidative stress, genotoxicity and histopathology) in Channa punctatus exposed to heavy metal loaded waste water

Javed, Mehjbeen, Ahmad, Md. Irshad, Usmani, Nazura, Ahmad, Masood

2017

Overview

Experiments were conducted to investigate the health of fish Channa punctatus inhabiting heavy metal-loaded waste water. Heavy metals in the order of Fe > Mn > Zn > Co > Ni > Cu = Cr were present in the waste water. Gills had high metal load followed by liver and then kidney. Albumin, albumin to globulin (A:G) ratio, triglyceride, high density lipoprotein (HDL) and very low density lipoprotein (VLDL) were found to be lower but phospholipid, low density lipoprotein (LDL), total protein, lipid and cholesterol were higher as compared to the reference. Oxidative stress markers such as superoxide dismutase (SOD), catalase (CAT), glutathione S transferase (GST) and lipid peroxidation (LPO) were significantly higher in all tissues, whereas reduced glutathione (GSH) levels were comparatively low. Damage to DNA was observed with significantly higher mean tail length of comets in the exposed fish gill cells (30.9 µm) followed by liver (24.3 µm) and kidney (20.6 µm) as compared to reference fish (5.2, 4.8 and 5.9 µm respectively). Histopathology in gill, liver and kidney also showed marked damage. Integrated biochemical, oxidative stress, genotoxicity and histopathological findings are valuable biomarkers for native fish adaptive patterns, and monitoring of water quality/pollution of freshwater ecosystems.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 631/601 / 704/172/4081 / Albumin / Animals / Aquatic ecosystems / Biomarkers - blood / Blood Glucose - metabolism / Catalase / Cholesterol / Chromium / Comet Assay / Copper / Deoxyribonucleic acid / DNA / DNA Damage / Environmental Exposure - analysis / Fish Proteins - blood / Fishes - blood / Freshwater ecosystems / Freshwater pollution / Genotoxicity / Gills / Gills - drug effects / Gills - metabolism / Gills - pathology / Globulins / Glutathione / Heavy metals / High density lipoprotein / Histopathology / Humanities and Social Sciences / Kidney - drug effects / Kidney - metabolism / Kidney - pathology / Kidneys / Lipid peroxidation / Lipids - blood / Lipoproteins (very low density) / Liver / Liver - drug effects / Liver - metabolism / Liver - pathology / Low density lipoprotein / Manganese / Metals, Heavy - toxicity / multidisciplinary / Mutagens - toxicity / Oxidative stress / Oxidative Stress - drug effects / Peroxidation / Phospholipids / Pollution monitoring / Science / Science (multidisciplinary) / Superoxide dismutase / Waste Water - chemistry / Wastewater / Water monitoring / Water Pollutants, Chemical - toxicity / Water Pollution / Water quality / Water quality management