Journal Article

Evaluation of oxidative stress, inflammation, apoptosis, oxidative DNA damage and metalloproteinases in the lungs of rats treated with cadmium and carvacrol

Yesildag, Kerim, Ileriturk, Mustafa, Kandemir, Fatih Mehmet, Gur, Cihan

2022

Overview

Background The potential protective properties of carvacrol (CRV), which possesses various biological and pharmacological properties, against lung toxicity caused by cadmium (Cd), a major environmental pollutant, were investigated in the present study. Methods and results In the study, rats were given 25 or 50 mg/kg CRV orally 30 min after administrating 25 mg/kg cadmium chloride for seven days. Subsequently, the levels of 8-OHdG, MMP-2, and MMP-9, as well as markers of oxidative stress, inflammation, and apoptosis, were analyzed in the lung tissue of the animals. The results revealed that CRV exhibited antioxidant characteristics and raised SOD, CAT, GPx, and CAT levels and decreased the MDA levels induced by Cd. It also suppressed proinflammatory cytokines by lowering the levels of CRV NF-κB and p38 MAPK, thus exerting an anti-inflammatory effect against Cd. It was found that the levels of Bax, Caspase-3, and cytochrome c increased by Cd were decreased by the application of CRV. CRV also showed an anti-apoptotic effect by increasing Bcl-2 levels. The levels of 8-OHdG, MMP2, and MMP9, which increased with Cd administration, were observed to reduce after treatment with CRV. Conclusions The results indicate that CRV has protective properties against Cd-induced lung toxicity.

• Publisher: Springer Netherlands,Springer Nature B.V
• Subject: 8-Hydroxydeoxyguanosine / Animal Anatomy / Animal Biochemistry / Animals / anti-inflammatory activity / Antioxidants / Antioxidants - metabolism / Apoptosis / Apoptosis - drug effects / Bcl-2 protein / Biomarkers - metabolism / Biomedical and Life Sciences / Cadmium / Cadmium - adverse effects / Cadmium - pharmacology / Cadmium chloride / Cadmium Chloride - metabolism / Carvacrol / Caspase-3 / Cell Line, Tumor / Cymenes - metabolism / Cymenes - pharmacology / Cytochrome c / Cytokines / DNA damage / DNA Damage - drug effects / DNA Damage - physiology / Gelatinase A / Gelatinase B / Histology / Inflammation / Inflammation - drug therapy / Inflammation - physiopathology / Kidney - metabolism / Life Sciences / Lipid Peroxidation - drug effects / Lung - metabolism / lungs / Male / MAP kinase / Metalloproteases - drug effects / Metalloproteases - metabolism / metalloproteinases / mitogen-activated protein kinase / molecular biology / Morphology / NF-kappa B - metabolism / NF-κB protein / Original Article / Oxidative stress / Oxidative Stress - drug effects / Oxidative Stress - physiology / Pollutants / Rats / Rats, Sprague-Dawley / Toxicity