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Oocytes with impaired meiotic maturation contain increased mtDNA deletions
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Journal Article
Oocytes with impaired meiotic maturation contain increased mtDNA deletions
2025
Overview
Purpose
Induction of meiotic competence is a major goal of the controlled ovarian stimulation used in ART. Do factors intrinsic to the oocyte contribute to oocyte maturation? Deletions in mtDNA accumulate in long-lived post mitotic tissues and are found in human oocytes. If oogenesis cleanses the germline of deleterious deletions in mtDNA, meiotically competent oocytes should contain lower levels of mtDNA deletions vs. meiotically arrested oocytes. We tested this hypothesis using a novel PCR assay for a deletion ratio in human oocytes derived from IVF.
Methods
A real-time PCR assay was developed to measure total mtDNA copy number (mtDNA
CN
) and mtDNA harboring the 5 Kb “common deletion” to enable calculation of the mtDNA deletion ratio (mtDNA
DR
) in 143 cultured oocytes. Kruskal-Wallis test was carried out to compare the total mtDNA
CN
and the mtDNA
DR
among oocytes which matured to metaphase II (MII) vs. oocytes arrested at GV or metaphase I (MI).
Results
51.75% of oocytes reached MII, and 17% remained at MI. Mean mtDNADR in GV, MI and MII oocytes were 27.87%, 31.88% and 20.05%, respectively. The difference in deletion ratios between GV and MII and between MI and MII stages was statistically significant
p
< 0.001 and
p
= 0.034, respectively. Additionally, patient age was found to be positively correlated with time to Polar body extrusion (− 0.278 Pearson correlation).
Conclusions
Oocytes with impaired meiotic maturation contain an increased load of mtDNA deletions. This is the first report of an association between the mtDNA deletion ratio and human oocyte maturation in vitro.
Publisher
Springer US,Springer Nature B.V
