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Vascular endothelial growth factor C mRNA expression is a prognostic factor in epithelial ovarian cancer as detected by kinetic RT-PCR in formalin-fixed paraffin-embedded tissue
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Vascular endothelial growth factor C mRNA expression is a prognostic factor in epithelial ovarian cancer as detected by kinetic RT-PCR in formalin-fixed paraffin-embedded tissue
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Vascular endothelial growth factor C mRNA expression is a prognostic factor in epithelial ovarian cancer as detected by kinetic RT-PCR in formalin-fixed paraffin-embedded tissue
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Journal Article
Vascular endothelial growth factor C mRNA expression is a prognostic factor in epithelial ovarian cancer as detected by kinetic RT-PCR in formalin-fixed paraffin-embedded tissue
2009
Overview
Vascular endothelial growth factor C (VEGF-C) is a well described chemotactic and growth factor for lymphatic endothelial cells. Its inhibition leads to suppression of lymphatic and distant metastases in mouse models. In ovarian cancer, the relationship between VEGF-C expression and tumor behavior has not yet been determined by a quantitative method
in vivo
. Therefore, we used a new technique of RNA extraction from formalin-fixed paraffin-embedded tissue samples and determined the expression levels of VEGF-C mRNA in a study group of 97 ovarian cancer patients. Expression levels were correlated with clinicopathological features and patient survival. High VEGF-C expression was associated with worse overall (
p
= 0.0393) and progression-free (
p
= 0.0155) patient survival. In the subgroups of serous tumors and high-grade tumors, VEGF-C mRNA was still a negative indicator for patient survival (
p
= 0.0190 and 0.0311, respectively). A trend was observed among patients with high clinical stage (
p
= 0.0634). In multivariate survival analysis VEGF-C mRNA retained its prognostic influence on progression-free survival (
p
= 0.006,
HR
= 0.319 with a 95% confidence interval of 0.142–0.720). High VEGF-C expression was further associated with an increased residual tumor mass after primary cytoreductive surgery. We found no correlation of VEGF-C expression with tumor grade, FIGO stage, lymph node, or distant metastases. Our study demonstrates that high VEGF-C expression is associated with aggressive tumor behavior in ovarian cancer. mRNA extracted from paraffin-embedded tumor samples is suitable for VEGF-C gene expression studies.
Publisher
Springer-Verlag,Springer,Springer Nature B.V
Subject
/ Aged
/ Biological and medical sciences
/ Female
/ Gene Expression Regulation, Neoplastic
/ Gynecology. Andrology. Obstetrics
/ Humans
/ Investigative techniques, diagnostic techniques (general aspects)
/ Medicine
/ Ovarian Neoplasms - genetics
/ Ovarian Neoplasms - metabolism
/ Ovarian Neoplasms - pathology
/ Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
/ RNA, Messenger - isolation & purification
/ Survival
/ Tumors
