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A fungal milRNA mediates epigenetic repression of a virulence gene in Verticillium dahliae
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A fungal milRNA mediates epigenetic repression of a virulence gene in Verticillium dahliae
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A fungal milRNA mediates epigenetic repression of a virulence gene in Verticillium dahliae
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Journal Article
A fungal milRNA mediates epigenetic repression of a virulence gene in Verticillium dahliae
2019
Overview
MiRNAs in animals and plants play crucial roles in diverse developmental processes under both normal and stress conditions. miRNA-like small RNAs (milRNAs) identified in some fungi remain functionally uncharacterized. Here, we identified a number of milRNAs in
Verticillium dahliae
, a soil-borne fungal pathogen responsible for devastating wilt diseases in many crops. Accumulation of a
V. dahliae
milRNA1, named VdmilR1, was detected by RNA gel blotting. We show that the precursor gene
VdMILR1
is transcribed by RNA polymerase II and is able to produce the mature VdmilR1, in a process independent of
V. dahliae
DCL (Dicer-like) and AGO (Argonaute) proteins. We found that an RNaseIII domain-containing protein, VdR3, is essential for
V. dahliae
and participates in VdmilR1 biogenesis. VdmilR1 targets a hypothetical protein-coding gene,
VdHy1
, at the 3′UTR for transcriptional repression through increased histone H3K9 methylation of
VdHy1
. Pathogenicity analysis reveals that
VdHy1
is essential for fungal virulence. Together with the time difference in the expression patterns of VdmilR1 and
VdHy1
during fungal infection in cotton plants, our findings identify a novel milRNA, VdmilR1, in
V. dahliae
synthesized by a noncanonical pathway that plays a regulatory role in pathogenicity and uncover an epigenetic mechanism for VdmilR1 in regulating a virulence target gene.
This article is part of the theme issue ‘Biotic signalling sheds light on smart pest management’.
Publisher
The Royal Society
