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In situ structure of the red algal phycobilisome–PSII–PSI–LHC megacomplex
by
Sun, Shan
, You, Xin
, Zhang, Xing
, Zhang, Xinzheng
, Cheng, Jing
, Ma, Jianfei
, Wang, Hong-Wei
, Sui, Sen-Fang
, Xiao, Yanan
in
101/28
/ 631/449/1734/2075
/ 631/449/1734/2076
/ 631/449/1734/2077
/ 631/535/1258/1259
/ 631/535/1258/1260
/ Algae
/ Antennas
/ Assembly
/ Carotenoids
/ Cryoelectron Microscopy
/ Cyanobacteria
/ Energy
/ Energy distribution
/ Energy Transfer
/ Humanities and Social Sciences
/ Light
/ Light-Harvesting Protein Complexes - chemistry
/ Light-Harvesting Protein Complexes - metabolism
/ Light-Harvesting Protein Complexes - ultrastructure
/ multidisciplinary
/ Photosynthesis
/ Photosystem I
/ Photosystem I Protein Complex - chemistry
/ Photosystem I Protein Complex - metabolism
/ Photosystem I Protein Complex - ultrastructure
/ Photosystem II
/ Photosystem II Protein Complex - chemistry
/ Photosystem II Protein Complex - metabolism
/ Photosystem II Protein Complex - ultrastructure
/ Phycobilisomes
/ Phycobilisomes - chemistry
/ Phycobilisomes - metabolism
/ Phycobilisomes - ultrastructure
/ Porphyridium - chemistry
/ Porphyridium - enzymology
/ Porphyridium - metabolism
/ Porphyridium - ultrastructure
/ Science
/ Science (multidisciplinary)
/ Single Molecule Imaging
/ Symmetry
/ Tomography
2023
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In situ structure of the red algal phycobilisome–PSII–PSI–LHC megacomplex
by
Sun, Shan
, You, Xin
, Zhang, Xing
, Zhang, Xinzheng
, Cheng, Jing
, Ma, Jianfei
, Wang, Hong-Wei
, Sui, Sen-Fang
, Xiao, Yanan
in
101/28
/ 631/449/1734/2075
/ 631/449/1734/2076
/ 631/449/1734/2077
/ 631/535/1258/1259
/ 631/535/1258/1260
/ Algae
/ Antennas
/ Assembly
/ Carotenoids
/ Cryoelectron Microscopy
/ Cyanobacteria
/ Energy
/ Energy distribution
/ Energy Transfer
/ Humanities and Social Sciences
/ Light
/ Light-Harvesting Protein Complexes - chemistry
/ Light-Harvesting Protein Complexes - metabolism
/ Light-Harvesting Protein Complexes - ultrastructure
/ multidisciplinary
/ Photosynthesis
/ Photosystem I
/ Photosystem I Protein Complex - chemistry
/ Photosystem I Protein Complex - metabolism
/ Photosystem I Protein Complex - ultrastructure
/ Photosystem II
/ Photosystem II Protein Complex - chemistry
/ Photosystem II Protein Complex - metabolism
/ Photosystem II Protein Complex - ultrastructure
/ Phycobilisomes
/ Phycobilisomes - chemistry
/ Phycobilisomes - metabolism
/ Phycobilisomes - ultrastructure
/ Porphyridium - chemistry
/ Porphyridium - enzymology
/ Porphyridium - metabolism
/ Porphyridium - ultrastructure
/ Science
/ Science (multidisciplinary)
/ Single Molecule Imaging
/ Symmetry
/ Tomography
2023
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In situ structure of the red algal phycobilisome–PSII–PSI–LHC megacomplex
by
Sun, Shan
, You, Xin
, Zhang, Xing
, Zhang, Xinzheng
, Cheng, Jing
, Ma, Jianfei
, Wang, Hong-Wei
, Sui, Sen-Fang
, Xiao, Yanan
in
101/28
/ 631/449/1734/2075
/ 631/449/1734/2076
/ 631/449/1734/2077
/ 631/535/1258/1259
/ 631/535/1258/1260
/ Algae
/ Antennas
/ Assembly
/ Carotenoids
/ Cryoelectron Microscopy
/ Cyanobacteria
/ Energy
/ Energy distribution
/ Energy Transfer
/ Humanities and Social Sciences
/ Light
/ Light-Harvesting Protein Complexes - chemistry
/ Light-Harvesting Protein Complexes - metabolism
/ Light-Harvesting Protein Complexes - ultrastructure
/ multidisciplinary
/ Photosynthesis
/ Photosystem I
/ Photosystem I Protein Complex - chemistry
/ Photosystem I Protein Complex - metabolism
/ Photosystem I Protein Complex - ultrastructure
/ Photosystem II
/ Photosystem II Protein Complex - chemistry
/ Photosystem II Protein Complex - metabolism
/ Photosystem II Protein Complex - ultrastructure
/ Phycobilisomes
/ Phycobilisomes - chemistry
/ Phycobilisomes - metabolism
/ Phycobilisomes - ultrastructure
/ Porphyridium - chemistry
/ Porphyridium - enzymology
/ Porphyridium - metabolism
/ Porphyridium - ultrastructure
/ Science
/ Science (multidisciplinary)
/ Single Molecule Imaging
/ Symmetry
/ Tomography
2023
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In situ structure of the red algal phycobilisome–PSII–PSI–LHC megacomplex
Journal Article
In situ structure of the red algal phycobilisome–PSII–PSI–LHC megacomplex
2023
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Overview
In oxygenic photosynthetic organisms, light energy is captured by antenna systems and transferred to photosystem II (PSII) and photosystem I (PSI) to drive photosynthesis
1
,
2
. The antenna systems of red algae consist of soluble phycobilisomes (PBSs) and transmembrane light-harvesting complexes (LHCs)
3
. Excitation energy transfer pathways from PBS to photosystems remain unclear owing to the lack of structural information. Here we present in situ structures of PBS–PSII–PSI–LHC megacomplexes from the red alga
Porphyridium purpureum
at near-atomic resolution using cryogenic electron tomography and in situ single-particle analysis
4
, providing interaction details between PBS, PSII and PSI. The structures reveal several unidentified and incomplete proteins and their roles in the assembly of the megacomplex, as well as a huge and sophisticated pigment network. This work provides a solid structural basis for unravelling the mechanisms of PBS–PSII–PSI–LHC megacomplex assembly, efficient energy transfer from PBS to the two photosystems, and regulation of energy distribution between PSII and PSI.
In situ structures of PBS–PSII–PSI–LHC megacomplexes from the alga
P. purpureum
at near-atomic resolution using cryogenic-electron tomography and in situ single-particle analysis are reported, providing interaction details between PBS, PSII and PSI.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ Algae
/ Antennas
/ Assembly
/ Energy
/ Humanities and Social Sciences
/ Light
/ Light-Harvesting Protein Complexes - chemistry
/ Light-Harvesting Protein Complexes - metabolism
/ Light-Harvesting Protein Complexes - ultrastructure
/ Photosystem I Protein Complex - chemistry
/ Photosystem I Protein Complex - metabolism
/ Photosystem I Protein Complex - ultrastructure
/ Photosystem II Protein Complex - chemistry
/ Photosystem II Protein Complex - metabolism
/ Photosystem II Protein Complex - ultrastructure
/ Phycobilisomes - ultrastructure
/ Porphyridium - ultrastructure
/ Science
/ Symmetry
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