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The Hybrid II assay: a sensitive and specific real-time hybridization assay for the diagnosis of Theileria parva infection in Cape buffalo (Syncerus caffer) and cattle
by
LATIF, ABDALLA A.
, THEKISOE, ORIEL M. M.
, POTGIETER, FRED T.
, PIENAAR, RONEL
, MANS, BEN J.
in
acute course
/ Animal helminthic diseases
/ Animals
/ Base Sequence
/ Biological and medical sciences
/ buffaloes
/ Buffaloes - parasitology
/ carrier state
/ Carrier State - veterinary
/ Cattle
/ cattle diseases
/ Coinfection - veterinary
/ control methods
/ Disease control
/ Helminthic diseases
/ Hybridization
/ Infectious diseases
/ Medical sciences
/ mixed infection
/ Molecular Sequence Data
/ nucleic acid hybridization
/ Nucleic Acid Hybridization - methods
/ Parasites
/ Parasitic diseases
/ polymerase chain reaction
/ Real-Time Polymerase Chain Reaction - veterinary
/ Sensitivity and Specificity
/ Sequence Alignment - veterinary
/ South Africa
/ Syncerus caffer
/ Theileria parva
/ Theileria parva - genetics
/ Theileria parva - isolation & purification
/ Theileriasis - diagnosis
/ Theileriasis - parasitology
/ theileriosis
2011
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The Hybrid II assay: a sensitive and specific real-time hybridization assay for the diagnosis of Theileria parva infection in Cape buffalo (Syncerus caffer) and cattle
by
LATIF, ABDALLA A.
, THEKISOE, ORIEL M. M.
, POTGIETER, FRED T.
, PIENAAR, RONEL
, MANS, BEN J.
in
acute course
/ Animal helminthic diseases
/ Animals
/ Base Sequence
/ Biological and medical sciences
/ buffaloes
/ Buffaloes - parasitology
/ carrier state
/ Carrier State - veterinary
/ Cattle
/ cattle diseases
/ Coinfection - veterinary
/ control methods
/ Disease control
/ Helminthic diseases
/ Hybridization
/ Infectious diseases
/ Medical sciences
/ mixed infection
/ Molecular Sequence Data
/ nucleic acid hybridization
/ Nucleic Acid Hybridization - methods
/ Parasites
/ Parasitic diseases
/ polymerase chain reaction
/ Real-Time Polymerase Chain Reaction - veterinary
/ Sensitivity and Specificity
/ Sequence Alignment - veterinary
/ South Africa
/ Syncerus caffer
/ Theileria parva
/ Theileria parva - genetics
/ Theileria parva - isolation & purification
/ Theileriasis - diagnosis
/ Theileriasis - parasitology
/ theileriosis
2011
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The Hybrid II assay: a sensitive and specific real-time hybridization assay for the diagnosis of Theileria parva infection in Cape buffalo (Syncerus caffer) and cattle
by
LATIF, ABDALLA A.
, THEKISOE, ORIEL M. M.
, POTGIETER, FRED T.
, PIENAAR, RONEL
, MANS, BEN J.
in
acute course
/ Animal helminthic diseases
/ Animals
/ Base Sequence
/ Biological and medical sciences
/ buffaloes
/ Buffaloes - parasitology
/ carrier state
/ Carrier State - veterinary
/ Cattle
/ cattle diseases
/ Coinfection - veterinary
/ control methods
/ Disease control
/ Helminthic diseases
/ Hybridization
/ Infectious diseases
/ Medical sciences
/ mixed infection
/ Molecular Sequence Data
/ nucleic acid hybridization
/ Nucleic Acid Hybridization - methods
/ Parasites
/ Parasitic diseases
/ polymerase chain reaction
/ Real-Time Polymerase Chain Reaction - veterinary
/ Sensitivity and Specificity
/ Sequence Alignment - veterinary
/ South Africa
/ Syncerus caffer
/ Theileria parva
/ Theileria parva - genetics
/ Theileria parva - isolation & purification
/ Theileriasis - diagnosis
/ Theileriasis - parasitology
/ theileriosis
2011
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The Hybrid II assay: a sensitive and specific real-time hybridization assay for the diagnosis of Theileria parva infection in Cape buffalo (Syncerus caffer) and cattle
Journal Article
The Hybrid II assay: a sensitive and specific real-time hybridization assay for the diagnosis of Theileria parva infection in Cape buffalo (Syncerus caffer) and cattle
2011
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Overview
Corridor disease is an acute, fatal disease of cattle caused by buffalo-adapted Theileria parva. This is a nationally controlled disease in South Africa and strict control measures apply for the movement of buffalo, which includes mandatory testing for the presence of T. parva and other controlled diseases. Accurate diagnosis of the T. parva carrier state in buffalo using the official real-time hybridization PCR assay (Sibeko et al. 2008), has been shown to be affected by concurrent infection with T. sp. (buffalo)-like parasites. We describe the Hybrid II assay, a real-time hybridization PCR method, which compares well with the official hybridization assay in terms of specificity and sensitivity. It is, however, not influenced by mixed infections of T. sp. (buffalo)-like parasites and is as such a significant improvement on the current hybridization assay.
Publisher
Cambridge University Press
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