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Lytic properties and genomic analysis of bacteriophage Brt_Psa3, targeting Pseudomonas syringae pv. actinidiae
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Lytic properties and genomic analysis of bacteriophage Brt_Psa3, targeting Pseudomonas syringae pv. actinidiae
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Journal Article
Lytic properties and genomic analysis of bacteriophage Brt_Psa3, targeting Pseudomonas syringae pv. actinidiae
2025
Overview
Pseudomonas syringae
pv.
actinidiae
(Psa) is the causative agent of bacterial canker in kiwifruit (
Actinidia
spp.). Psa biovar 3 is the most prevalent and virulent, causing frequent and severe outbreaks worldwide. While current treatments have low efficacy, bacteriophages emerge as possible environmentally safe alternative biocontrol agents. In this study, bacteriophage Brt_Psa3 was isolated from the soil of a kiwifruit orchard in Portugal. Morphologically, Brt_Psa3 forms clear plaques and has a Podoviral morphotype. The bacteriophage exhibited broad lytic activity against several plant-pathogenic
Pseudomonas
strains, including Psa isolates. The isolated bacteriophage has a latent period of 100 min, a burst size of 143 particles/cell, and demonstrates stability at different temperatures and pH values found in kiwifruit orchards. In addition, Brt_Psa3 exhibited tolerance to UVA irradiation during 120 min of incubation. Brt_Psa3 belongs to the
Autographiviridae
family and
Ghunavirus
genus, based on full-genome nucleotide alignment and supported by phylogenetic analysis of structural proteins. The phage contains 51 open reading frames with no antibiotic resistance genes identified, within a genome of 40.509 base pairs. In vitro experiments with kiwifruit leaves demonstrated significant reduction of Psa levels (40%) on leaf surfaces, highlighting the bacteriophage’s therapeutic potential in managing bacterial canker in kiwifruits.
Keypoints
•
Isolated bacteriophage Brt_Psa3 effectively infect Psa strains
•
Brt_Psa3 maintains infectivity under conditions similar to kiwifruit orchard environment
•
Leaf assay confirms its biocontrol potential
Publisher
Springer Berlin Heidelberg,Springer Nature B.V,Springer
Subject
/ Bacteria
/ Biomedical and Life Sciences
/ Canker
/ Copper
/ Genomes
/ Genomics
/ Leaves
/ Microbial Genetics and Genomics
/ Phages
/ Plant Diseases - microbiology
/ Plant Diseases - prevention & control
/ Podoviridae - classification
/ Podoviridae - isolation & purification
/ Pseudomonas Phages - classification
/ Pseudomonas Phages - genetics
/ Pseudomonas Phages - isolation & purification
/ Pseudomonas Phages - physiology
/ Pseudomonas Phages - ultrastructure
/ Pseudomonas syringae - virology
/ Pseudomonas syringae pv. actinidiae
