Journal Article

Antifungal and Antioxidant Properties of Chitosan Polymers Obtained from Nontraditional Polybius henslowii Sources

Cotrim Marques, Sónia, Pedrosa, Rui, Avelelas, Francisco, Marques Nunes, Paulo, Leandro, Sérgio Miguel, Pinto, Luís F.V., Horta, André

2019

Overview

Chitin was extracted from Polybius henslowii, a swimming crab, captured in large quantities throughout the Portuguese coast by purse seine vessels as bycatch. After standard chitin extraction procedures, water-soluble chitosan products were obtained via two different methods: (1) N-acetylation with the addition of acetic anhydride and (2) a reaction with hydrogen peroxide. The chemical structure and molecular weight of chitosan derivatives, water-soluble chitosan (WSC) and chitooligosaccharides (COS), were confirmed by Fourier Transform Infrared Spectroscopy (FT-IR) and gel permeation chromatography (GPC). Antioxidant and metal chelation activities were evaluated, and the growth inhibition capacity was tested on four phytopatogens. The chitooligosaccharides from pereopods (pCOS) and shell body parts (sCOS) inhibited all fungal species tested, particularly Cryphonectria parasitica with 84.7% and 85.5%, respectively. Both radical scavenging and antifungal activities proved to be dose-dependent. Chitooligosaccharides with a low molecular weight (2.7, 7.4, and 10.4 Kg·mol−1) showed the highest activity among all properties tested. These results suggested that chitosan derivatives from P. henslowii raw material could potentially be used against phytopathogens or as ingredient in cosmetics and other products related to oxidative stress.

• Publisher: MDPI AG,MDPI
• Subject: Acetic anhydride / Acetylation / Analytical methods / Animals / Antifungal activity / Antifungal agents / Antifungal Agents - chemistry / Antifungal Agents - pharmacology / antioxidant activity / Antioxidant properties / Antioxidants / Antioxidants - chemistry / Antioxidants - pharmacology / Body parts / Brachyura - chemistry / Bycatch / Chelation / Chitin / Chitin - analogs & derivatives / Chitin - chemistry / Chitin - pharmacology / chitooligosaccharides / Chitosan / Chitosan - chemistry / Chitosan - pharmacology / Cosmetics / Crustaceans / Extraction procedures / Fourier transforms / Fungi - drug effects / Fungicides / Gels / Hydrogen / Hydrogen peroxide / Hydrogen Peroxide - pharmacology / Infrared spectroscopy / Lipids / Liquid chromatography / Low molecular weights / Marine crustaceans / marine resources / Metals / Molecular structure / Molecular Weight / Oxidative stress / Pathogens / Polybius / Polybius henslowii / Polymers / Polymers - chemistry / Polymers - pharmacology / Properties / Proteins / Purse seines / Raw materials / Scavenging / Solubility / Spectroscopy, Fourier Transform Infrared - methods / Swimming / Viscosity / Water / Water chemistry