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Discovery of nitrate–CPK–NLP signalling in central nutrient–growth networks
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Discovery of nitrate–CPK–NLP signalling in central nutrient–growth networks
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Journal Article
Discovery of nitrate–CPK–NLP signalling in central nutrient–growth networks
2017
Overview
Nutrient signalling integrates and coordinates gene expression, metabolism and growth. However, its primary molecular mechanisms remain incompletely understood in plants and animals. Here we report unique Ca
2+
signalling triggered by nitrate with live imaging of an ultrasensitive biosensor in
Arabidopsis
leaves and roots. A nitrate-sensitized and targeted functional genomic screen identifies subgroup III Ca
2+
-sensor protein kinases (CPKs) as master regulators that orchestrate primary nitrate responses. A chemical switch with the engineered mutant CPK10(M141G) circumvents embryo lethality and enables conditional analyses of
cpk10 cpk30 cpk32
triple mutants to define comprehensive nitrate-associated regulatory and developmental programs. Nitrate-coupled CPK signalling phosphorylates conserved NIN-LIKE PROTEIN (NLP) transcription factors to specify the reprogramming of gene sets for downstream transcription factors, transporters, nitrogen assimilation, carbon/nitrogen metabolism, redox, signalling, hormones and proliferation. Conditional
cpk10 cpk30 cpk32
and
nlp7
mutants similarly impair nitrate-stimulated system-wide shoot growth and root establishment. The nutrient-coupled Ca
2+
signalling network integrates transcriptome and cellular metabolism with shoot–root coordination and developmental plasticity in shaping organ biomass and architecture.
In response to nitrate, Ca
2+
-sensor protein kinases (CPKs) act as master regulators to coordinate downstream signalling responses that are essential for shoot growth and root establishment in
Arabidopsis
.
How nitrates stimulate roots and shoots
In plants, nutrient-associated signals affect gene expression, metabolism and ultimately growth, yet many of the molecular components connecting these signals to gene expression remain unknown. Jen Sheen and her team report that, in response to nitrate, the Ca
2+
-sensor protein kinases (CPKs) act as master regulators to coordinate downstream responses. Specifically, CPK phosphorylates the NIN-like protein (NLP) transcription factor, resulting in the reprogramming of genes that encode additional transcription factors, transporters and proteins that regulate nitrate metabolism, among others. This pathway is essential for nitrate-stimulated shoot growth and root establishment.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 14/63
/ 38
/ 38/22
/ 38/23
/ 38/39
/ 38/77
/ 38/90
/ 45
/ Amidohydrolases - metabolism
/ Arabidopsis - growth & development
/ Arabidopsis Proteins - chemistry
/ Arabidopsis Proteins - genetics
/ Arabidopsis Proteins - metabolism
/ Biomass
/ Calcium
/ Calcium-Binding Proteins - chemistry
/ Calcium-Binding Proteins - genetics
/ Calcium-Binding Proteins - metabolism
/ Calcium-Calmodulin-Dependent Protein Kinases - chemistry
/ Calcium-Calmodulin-Dependent Protein Kinases - genetics
/ Calcium-Calmodulin-Dependent Protein Kinases - metabolism
/ Food
/ Gene Expression Regulation, Plant
/ Hormones
/ Humanities and Social Sciences
/ Kinases
/ Leaves
/ Nitrates
/ Nitrogen
/ Plant Roots - growth & development
/ Plant Shoots - growth & development
/ Plants, Genetically Modified
/ Protein Kinases - metabolism
/ Proteins
/ Science
