Journal Article

Dual-Driven Hemostats Featured with Puncturing Erythrocytes for Severe Bleeding in Complex Wounds

Lu, Fei, Shou, Dahua, Ding, Weiwei, Wang, Xinyu, Guangqian Lan, Qiu, Haoyu, Hu, Enling, Xie, Ruiqi, Yu, Kun, Wang, Wenyi, Shang, Songmin

2022

Overview

Achieving rapid hemostasis in complex and deep wounds with secluded hemorrhagic sites is still a challenge because of the difficulty in delivering hemostats to these sites. In this study, a Janus particle, SEC-Fe@CaT with dual-driven forces, bubble-driving, and magnetic field– (MF–) mediated driving, was prepared via in situ loading of Fe 3 O 4 on a sunflower sporopollenin exine capsule (SEC), and followed by growth of flower-shaped CaCO 3 clusters. The bubble-driving forces enabled SEC-Fe@CaT to self-diffuse in the blood to eliminate agglomeration, and the MF-mediated driving force facilitated the SEC-Fe@CaT countercurrent against blood to access deep bleeding sites in the wounds. During the movement in blood flow, the meteor hammer-like SEC from SEC-Fe@CaT can puncture red blood cells (RBCs) to release procoagulants, thus promoting activation of platelet and rapid hemostasis. Animal tests suggested that SEC-Fe@CaT stopped bleeding in as short as 30 and 45 s in femoral artery and liver hemorrhage models, respectively. In contrast, the similar commercial product Celox™ required approximately 70 s to stop the bleeding in both bleeding modes. This study demonstrates a new hemostat platform for rapid hemostasis in deep and complex wounds. It was the first attempt integrating geometric structure of sunflower pollen with dual-driven movement in hemostasis.

• Subject: Bleeding / Blood flow / Erythrocytes / Femoral artery / Helianthus / Hemorrhage / Hemostasis / Hemostatics / Iron oxides / Sunflowers / Wounds