Journal Article

Pretreatment with IL-15 and IL-18 rescues natural killer cells from granzyme B-mediated apoptosis after cryopreservation

Berjis, Abdulla, Johnson, Omar, Chen, Linhui, Sheppard, Neil C., Aguilar, Oscar A., Muthumani, Deeksha, Finck, Amanda V., June, Carl H., Pomp, Oz, Plachta, Nicolas, Scholler, John, Lanier, Lewis L., Engel, Nils W.

2024

Overview

Human natural killer (NK) cell-based therapies are under assessment for treating various cancers, but cryopreservation reduces both the recovery and function of NK cells, thereby limiting their therapeutic feasibility. Using cryopreservation protocols optimized for T cells, here we find that ~75% of NK cells die within 24 h post-thaw, with the remaining cells displaying reduced cytotoxicity. Using CRISPR-Cas9 gene editing and confocal microscopy, we find that cryopreserved NK cells largely die via apoptosis initiated by leakage of granzyme B from cytotoxic vesicles. Pretreatment of NK cells with a combination of Interleukins-15 (IL-15) and IL-18 prior to cryopreservation improves NK cell recovery to ~90-100% and enables equal tumour control in a xenograft model of disseminated Raji cell lymphoma compared to non-cryopreserved NK cells. The mechanism of IL-15 and IL-18-induced protection incorporates two mechanisms: a transient reduction in intracellular granzyme B levels via degranulation, and the induction of antiapoptotic genes. Natural killer (NK) cells are assessed for various therapies, but sub-optimal cryopreservation dampens their clinical feasibility. Here the authors show that pretreating human NK cells with IL-15/IL-18 prior to cryopreservation improves NK cell post-thaw viability and functions, potentially via anti-apoptosis gene induction and granzyme B degranulation.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 13/106 / 13/2 / 13/31 / 14/19 / 38 / 42/41 / 49/91 / 631/250/127/1213 / 631/250/1932 / 631/250/2504/2506 / 692/4028/67/1059/2325 / Animals / Apoptosis / Cell Line, Tumor / Confocal microscopy / CRISPR / CRISPR-Cas Systems / Cryopreservation / Cryopreservation - methods / Cytotoxicity / Degranulation / Feasibility / Genetic modification / Genome editing / Granzyme B / Granzymes - metabolism / Humanities and Social Sciences / Humans / Interleukin 15 / Interleukin 18 / Interleukin-15 - pharmacology / Interleukin-18 - pharmacology / Killer Cells, Natural - cytology / Killer Cells, Natural - metabolism / Lymphocytes / Lymphocytes T / Lymphoma / Mice / multidisciplinary / Natural killer cells / Pretreatment / Recovery / Science / Science (multidisciplinary) / Xenotransplantation