Asset Details
Do you wish to reserve the book?
Flow Cytometric Analysis of Macrophages and Dendritic Cell Subsets in the Mouse Lung
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Flow Cytometric Analysis of Macrophages and Dendritic Cell Subsets in the Mouse Lung
Do you wish to request the book?
Flow Cytometric Analysis of Macrophages and Dendritic Cell Subsets in the Mouse Lung
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Flow Cytometric Analysis of Macrophages and Dendritic Cell Subsets in the Mouse Lung
2013
Overview
The lung hosts multiple populations of macrophages and dendritic cells, which play a crucial role in lung pathology. The accurate identification and enumeration of these subsets are essential for understanding their role in lung pathology. Flow cytometry is a mainstream tool for studying the immune system. However, a systematic flow cytometric approach to identify subsets of macrophages and dendritic cells (DCs) accurately and consistently in the normal mouse lung has not been described. Here we developed a panel of surface markers and an analysis strategy that accurately identify all known populations of macrophages and DCs, and their precursors in the lung during steady-state conditions and bleomycin-induced injury. Using this panel, we assessed the polarization of lung macrophages during the course of bleomycin-induced lung injury. Alveolar macrophages expressed markers of alternatively activated macrophages during both acute and fibrotic phases of bleomycin-induced lung injury, whereas markers of classically activated macrophages were expressed only during the acute phase. Taken together, these data suggest that this flow cytometric panel is very helpful in identifying macrophage and DC populations and their state of activation in normal, injured, and fibrotic lungs.
Publisher
American Thoracic Society
Subject
/ Dendritic Cells - drug effects
/ Dendritic Cells - metabolism
/ Lung Injury - chemically induced
/ Macrophages, Alveolar - cytology
/ Macrophages, Alveolar - drug effects
/ Macrophages, Alveolar - metabolism
/ Macrophages, Alveolar - pathology
/ Male
/ Mice
/ Myeloid Cells - drug effects
/ Pulmonary Fibrosis - chemically induced
