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Ecdysone signaling-induced dumpless1 expression controls nurse cell dumping in Drosophila oogenesis
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Ecdysone signaling-induced dumpless1 expression controls nurse cell dumping in Drosophila oogenesis
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Journal Article
Ecdysone signaling-induced dumpless1 expression controls nurse cell dumping in Drosophila oogenesis
2025
Overview
Nurse cell (NC) dumping, a process essential for oocyte development, involves the rapid cytoplasmic transfer from germline-derived NCs into the oocyte. However, its regulatory mechanism remains unclear. Here, we report that ecdysone signaling in stretch follicle cells (SFCs) regulates NC dumping through
dumpless1
, a ZAD-C
2
H
2
zinc finger transcription factor, in
Drosophila
. Ecdysone induced
dumpless1
expression in SFCs, and CRISPR/Cas9-mediated knockout of
dumpless1
or its functional domain ZAD suppresses NC dumping. Depletion of
dumpless1
upregulates integrin βPS expression in SFC plasma membrane, while reducing cortical enrichment of Rho1 signaling-dependent phosphorylated myosin light chain (p-MLC) and disrupting actin cables organization in NCs. SFC-specific overexpression of integrin βPS reduces p-MLC enrichment in the NC cortex, whereas its knockdown in SFCs of
dumpless1
-/-
mutants partially rescues NC dumping defect. Our findings identify
dumpless1
as a critical effector of ecdysone signaling, bridging somatic-germline communication through the integrin βPS-Rho1-p-MLC axis, revealing a multicellular regulatory mechanism in
Drosophila
oogenesis.
Li et al. identify a previously uncharacterized transcription factor
dumpless1
as a critical effector of ecdysone signaling in stretch follicle cell, bridging somatic-germline communication through the integrin βPS-Rho1-p-MLC axis to facilitate dumping.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 13/106
/ 13/109
/ 13/44
/ 13/89
/ 14/19
/ 14/35
/ 14/63
/ 38
/ 38/77
/ 49/39
/ Actin
/ Animals
/ Cables
/ CRISPR
/ Cysts
/ Drosophila melanogaster - genetics
/ Drosophila melanogaster - metabolism
/ Drosophila Proteins - genetics
/ Drosophila Proteins - metabolism
/ Dumping
/ Ecdysone
/ Eggs
/ Female
/ Humanities and Social Sciences
/ Insects
/ Integrin beta Chains - genetics
/ Integrin beta Chains - metabolism
/ Myosin
/ Ovarian Follicle - metabolism
/ Ovaries
/ Regulatory mechanisms (biology)
/ rho GTP-Binding Proteins - genetics
/ rho GTP-Binding Proteins - metabolism
/ Science
/ Transcription Factors - genetics
