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VP6-sequence-based cutoff values as a criterion for rotavirus species demarcation
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VP6-sequence-based cutoff values as a criterion for rotavirus species demarcation
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Journal Article
VP6-sequence-based cutoff values as a criterion for rotavirus species demarcation
2012
Overview
Indirect immunofluorescence techniques targeting the rotavirus (RV) protein VP6 are used to differentiate RV species. The ICTV recognizes RV species A to E and two tentative species, F and G. A potential new RV species, ADRV-N, has been described. Phylogenetic trees and pairwise identity frequency graphs were constructed with more than 400 available VP6 sequences and seven newly determined VP6 sequences of RVD strains. All RV species were separated into distinct phylogenetic clusters. An amino acid sequence cutoff value of 53% firmly permitted differentiation of RV species, and ADRV-N was tentatively assigned to a novel RV species H (RVH).
Publisher
Springer Vienna,Springer,Springer Nature B.V
Subject
/ Animals
/ Biological and medical sciences
/ Biomedical and Life Sciences
/ Chickens
/ fluorescent antibody technique
/ Fundamental and applied biological sciences. Psychology
/ genetics
/ Genomes
/ Graphs
/ Poultry
/ proteins
/ R&D
/ Rotavirus - isolation & purification
/ Rotavirus Infections - veterinary
/ Rotavirus Infections - virology
/ Sequence Homology, Amino Acid
/ Serology
/ Software
/ Virology
/ Viruses
