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PyWRKY26 and PybHLH3 cotargeted the PyMYB114 promoter to regulate anthocyanin biosynthesis and transport in red-skinned pears
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PyWRKY26 and PybHLH3 cotargeted the PyMYB114 promoter to regulate anthocyanin biosynthesis and transport in red-skinned pears
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Journal Article
PyWRKY26 and PybHLH3 cotargeted the PyMYB114 promoter to regulate anthocyanin biosynthesis and transport in red-skinned pears
2020
Overview
Red pear is favored because of its bright appearance and abundant anthocyanins. Anthocyanin biosynthesis is controlled by transcription factors (TFs) forming regulatory complexes. In red-skinned pears, the WRKY TFs have a significant relationship with anthocyanin biosynthesis, but the molecular mechanism of the WRKY TFs involved in regulating color formation in red-skinned pear is unclear. In this study, the TFs
PyWRKY31
and
PyWRKY26
were screened as candidate genes for controlling anthocyanin biosynthesis by transcriptome data and bioinformatics analysis. The effect of anthocyanin accumulations after cotransformation of
PyWRKY31
or
PyWRKY26
with its partners
PyMYB10
,
PyMYB114
, and
PybHLH3
was verified in tobacco leaves and strawberry receptacles by a transient expression system. RT-qPCR analysis and a dual-luciferase reporter system further confirmed that this cotransformation activated the expression of
PyDFR
,
PyANS
, and
PyUFGT
in anthocyanin biosynthesis and
PyGST
in anthocyanin transport instead of the
PyABC transporter
and
PyAVP
. Furthermore, the cotransformed
PyWRKY26
and
PybHLH3
could bind to the
PyMYB114
promoter, and
PyWRKY26
directly activated the transcription of
PyMYB114
. In addition, the TF PyWRKY26 could interact with PybHLH3, as confirmed by firefly luciferase complementation and yeast two-hybrid (Y2H) assays. These results showed that the interaction of PyWRKY26 and PybHLH3 could cotarget the
PyMYB114
promoter, which resulted in anthocyanin accumulation in red-skinned pear. This study further strengthened the understanding of the regulatory mechanism of anthocyanin accumulation and contributed to improving the appearance of red-skinned pears.
Publisher
Nature Publishing Group UK,Oxford University Press
Subject
