Journal Article

Colorimetric determination of glutathione by using a nanohybrid composed of manganese dioxide and carbon dots

Chi, Yuwu, Wang, Qian, Pang, Hongchang, Dong, Yongqiang, Fu, Fengfu

2018

Overview

A kind of single-layer carbon based dots (CDs) with abundant carboxyl functional groups was hybridized with manganese dioxide (MnO 2 ). The resulting nanohybrid is stable and can be well dispersed in water. MnO 2 is capable of oxidizing the substrate 3,3′5,5′-tetramethylbenzidine (TMB) to form a blue product whose absorption (peaking at 655 nm) fades in the presence of glutathione (GSH). A sensitive and selective colorimetric GSH assay was worked out that has a linear response in the 10 to 0.1 µM GSH concentration range, with a 0.095 μM detection limit. The method was applied to the determination of GSH in spiked fetal calf serum where it gave excellent recoveries. Graphical abstract Schematic of the preparation of a nanohybrid composed of manganese dioxide and carbon based dots (MnO 2 /CDs). They can be used for the colorimetric detection of glutathione (GSH) based on the color change of 3,3′5,5′-tetramethylbenzidine (TMB).

• Publisher: Springer Vienna,Springer,Springer Nature B.V
• Subject: Analytical Chemistry / Carbon / Carbon dots / Characterization and Evaluation of Materials / Chemistry / Chemistry and Materials Science / Colorimetry / Enzymes / Fetal calf serum / Fourier transforms / Functional groups / Glutathione / Investigations / Manganese / Manganese dioxide / Microengineering / Nanochemistry / Nanomaterials / Nanotechnology / Optical properties / Original Paper / Oxidation / Spectrum analysis / Substrates