Journal Article

The Arabidopsis male-sterile mutant dde2-2 is defective in the ALLENE OXIDE SYNTHASE gene encoding one of the key enzymes of the jasmonic acid biosynthesis pathway

von Malek, Bernadette, Schneitz, Kay, Keller, Beat, van der Graaff, Eric

2002

Overview

The Arabidopsis thaliana (L.) Heynh. mutant delayed-dehiscence2-2 (dde2-2) was identified in an En1/Spm1 transposon-induced mutant population screened for plants showing defects in fertility. The dde2-2 mutant allele is defective in the anther dehiscence process and filament elongation and thus exhibits a male-sterile phenotype. The dde2-2 phenotype can be rescued by application of methyl jasmonate, indicating that the mutant is affected in jasmonic acid biosynthesis. The combination of genetic mapping and a candidate-gene approach identified a frameshift mutation in the ALLENE OXIDE SYNTHASE (AOS) gene, encoding one of the key enzymes of jasmonic acid biosynthesis. Expression analysis and genetic complementation of the dde2-2 phenotype by overexpression of the AOS coding sequence confirmed that the male-sterile phenotype is indeed caused by the mutation in the AOS gene.

• Publisher: Springer-Verlag,Springer,Springer Nature B.V
• Subject: Acetates / Acetates - pharmacology / Agronomy. Soil science and plant productions / Alleles / Amino Acid Sequence / amino acid sequences / Anthers / AOS gene / Arabidopsis / Arabidopsis - drug effects / Arabidopsis - enzymology / Arabidopsis - genetics / Arabidopsis thaliana / biochemical pathways / Biological and medical sciences / Biosynthesis / Chromosome Mapping / Chromosomes, Plant / Chromosomes, Plant - genetics / Complementation / Cyclopentanes / Cyclopentanes - metabolism / Cyclopentanes - pharmacology / Dehiscence / drug effects / Elongation / Enzymes / enzymology / fecundity / Fertility / Fertility - genetics / Filaments / Flower buds / Frameshift mutation / Fundamental and applied biological sciences. Psychology / Gene mapping / genes / genetic complementation / Genetic Complementation Test / Genetic mutation / genetics / Genetics and breeding of economic plants / Heterosis. Floral biology applications: apomixy, male sterility, incompatibility, varia / Intramolecular Oxidoreductases / Intramolecular Oxidoreductases - genetics / Intramolecular Oxidoreductases - metabolism / Jasmonic acid / metabolism / Methyl jasmonate / Molecular Sequence Data / Mutants / Mutation / Oxides / Oxylipins / pharmacology / Phenotypes / Plant breeding: fundamental aspects and methodology / Plant physiology and development / Plants / Pollen / pollination / Sequence Homology, Amino Acid / Sexual reproduction / Vegetative and sexual reproduction, floral biology, fructification