Journal Article

Discovery of nitrate-CPK-NLP signalling in central nutrient-growth networks

Mccormack, Matthew, Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)) ; Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Basic Forestry and Proteomics Research Cente ; Fujian Agriculture and Forestry University (FAFU), Shokat, Kevan, Department of Molecular Biology and Centre for Computational and Integrative Biology ; Massachusetts General Hospital [Boston], Yanagisawa, Shuichi, Boudsocq, Marie, Department Cellular and Molecular Pharmacology ; University of California [San Francisco] (UC San Francisco) ; University of California (UC)-University of California (UC), Ishida, Tetsuya, Niu, Yajie, Howard Hughes Medical Institute and Department ; University of California [San Francisco] (UC San Francisco) ; University of California (UC)-University of California (UC), Maekawa, Shugo, Department of Genetics ; The

2017

Overview

Nutrient signalling integrates and coordinates gene expression, metabolism and growth. However, its primary molecular mechanisms remain incompletely understood in plants and animals. Here we report unique Ca2+ signalling triggered by nitrate with live imaging of an ultrasensitive biosensor in Arabidopsis leaves and roots. A nitrate-sensitized and targeted functional genomic screen identifies subgroup III Ca2+-sensor protein kinases (CPKs) as master regulators that orchestrate primary nitrate responses. A chemical switch with the engineered mutant CPK10(M141G) circumvents embryo lethality and enables conditional analyses of cpk10 cpk30 cpk32 triple mutants to define comprehensive nitrate-associated regulatory and developmental programs. Nitrate-coupled CPK signalling phosphorylates conserved NIN-LIKE PROTEIN (NLP) transcription factors to specify the reprogramming of gene sets for downstream transcription factors, transporters, nitrogen assimilation, carbon/nitrogen metabolism, redox, signalling, hormones and proliferation. Conditional cpk10 cpk30 cpk32 and nlp7 mutants similarly impair nitrate-stimulated system-wide shoot growth and root establishment. The nutrient-coupled Ca2+ signalling network integrates transcriptome and cellular metabolism with shoot-root coordination and developmental plasticity in shaping organ biomass and architecture.

• Publisher: Nature Publishing Group,CCSD,Nature Publishing Group UK
• Subject: 14/19 / 14/63 / 38 / 38/22 / 38/23 / 38/39 / 38/77 / 38/90 / 45 / 631/449/2653 / 631/449/2675 / Amidohydrolases - genetics / Amidohydrolases - metabolism / Amino Acid Sequence / Arabidopsis - genetics / Arabidopsis - growth & development / Arabidopsis - metabolism / Arabidopsis Proteins - chemistry / Arabidopsis Proteins - genetics / Arabidopsis Proteins - metabolism / Biomass / Biosensors / Calcium / Calcium - metabolism / Calcium ions / Calcium nitrate / Calcium Signaling / Calcium signalling / Calcium-Binding Proteins - chemistry / Calcium-Binding Proteins - genetics / Calcium-Binding Proteins - metabolism / Calcium-Calmodulin-Dependent Protein Kinases - chemistry / Calcium-Calmodulin-Dependent Protein Kinases - genetics / Calcium-Calmodulin-Dependent Protein Kinases - metabolism / Carbon - metabolism / Cellular communication / Cellular Reprogramming / Developmental plasticity / Flowers & plants / Food / Gene expression / Gene Expression Regulation, Plant / Hormones / Humanities and Social Sciences / Kinases / Leaves / Lethality / Life Sciences / Metabolism / Molecular modelling / multidisciplinary / Nitrates / Nitrates - metabolism / Nitrogen / Nitrogen - metabolism / Nutrients / Oxidation-Reduction / Phosphorylation / Plant Roots - growth & development / Plant Roots - metabolism / Plant Shoots - growth & development / Plant Shoots - metabolism / Plants, Genetically Modified / Plastic foam / Protein kinase / Protein Kinases - chemistry / Protein Kinases - genetics / Protein Kinases - metabolism / Proteins / Science / Signal Transduction / Signaling / Subgroups / Transcription factors / Transcription, Genetic / Transcriptome
• ISBN: 0004014665000