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A compendium of DNA-binding specificities of transcription factors in Pseudomonas syringae
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A compendium of DNA-binding specificities of transcription factors in Pseudomonas syringae
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Journal Article
A compendium of DNA-binding specificities of transcription factors in Pseudomonas syringae
2020
Overview
Pseudomonas syringae
is a Gram-negative and model pathogenic bacterium that causes plant diseases worldwide. Here, we set out to identify binding motifs for all 301 annotated transcription factors (TFs) of
P. syringae
using HT-SELEX. We successfully identify binding motifs for 100 TFs. We map functional interactions between the TFs and their targets in virulence-associated pathways, and validate many of these interactions and functions using additional methods such as ChIP-seq, electrophoretic mobility shift assay (EMSA), RT-qPCR, and reporter assays. Our work identifies 25 virulence-associated master regulators, 14 of which had not been characterized as TFs before.
The authors set out to identify binding motifs for all 301 transcription factors of a plant pathogenic bacterium,
Pseudomonas syringae
, using HT-SELEX. They successfully identify binding motifs for 100 transcription factors, infer their binding sites on the genome, and validate the predicted interactions and functions.
