Journal Article

HIV-1 capsids enter the FG phase of nuclear pores like a transport receptor

Swanson, Nicholas A., Fu, Liran, Akkermans, Onno, Görlich, Dirk, Schwartz, Thomas U., Weiskopf, Erika N., Cheng, Shiya

2024

Overview

HIV-1 infection requires nuclear entry of the viral genome. Previous evidence suggests that this entry proceeds through nuclear pore complexes (NPCs), with the 120 × 60 nm capsid squeezing through an approximately 60-nm-wide central channel 1 and crossing the permeability barrier of the NPC. This barrier can be described as an FG phase 2 that is assembled from cohesively interacting phenylalanine–glycine (FG) repeats 3 and is selectively permeable to cargo captured by nuclear transport receptors (NTRs). Here we show that HIV-1 capsid assemblies can target NPCs efficiently in an NTR-independent manner and bind directly to several types of FG repeats, including barrier-forming cohesive repeats. Like NTRs, the capsid readily partitions into an in vitro assembled cohesive FG phase that can serve as an NPC mimic and excludes much smaller inert probes such as mCherry. Indeed, entry of the capsid protein into such an FG phase is greatly enhanced by capsid assembly, which also allows the encapsulated clients to enter. Thus, our data indicate that the HIV-1 capsid behaves like an NTR, with its interior serving as a cargo container. Because capsid-coating with trans -acting NTRs would increase the diameter by 10 nm or more, we suggest that such a ‘self-translocating’ capsid undermines the size restrictions imposed by the NPC scaffold, thereby bypassing an otherwise effective barrier to viral infection. The HIV-1 capsid behaves like a nuclear transport receptor entering and traversing an FG phase, with its interior serving as a cargo container, bypassing an otherwise effective barrier to viral infection.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 14/19 / 631/326/596/1787 / 631/80/389/2333 / 82/103 / 82/81 / Active Transport, Cell Nucleus / Capsid - chemistry / Capsid - metabolism / Capsid protein / Capsid Proteins - chemistry / Capsid Proteins - metabolism / Capsids / Cargo containers / Channel pores / Cohesion / DNA probes / Genomes / Glycine / Glycine - metabolism / HIV / HIV-1 - chemistry / HIV-1 - genetics / HIV-1 - metabolism / Human immunodeficiency virus / Humanities and Social Sciences / Humans / Infections / multidisciplinary / Nuclear Pore - chemistry / Nuclear Pore - metabolism / Nuclear Pore - virology / Nuclear Pore Complex Proteins - chemistry / Nuclear Pore Complex Proteins - metabolism / Nuclear pores / Nuclear transport / Permeability / Phenylalanine / Phenylalanine - metabolism / Proteins / Receptors / Science / Science (multidisciplinary) / Solubility / Viral infections / Virus Internalization