Journal Article

HLA dosage effect in narcolepsy with cataplexy

Claas, Frans H. J., van der Heide, Astrid, Drabbels, Jos J. M., Lammers, Gert J., Verduijn, Willem, Haasnoot, Geert W.

2015

Overview

Narcolepsy with cataplexy is a sleep disorder caused by the loss of hypocretin-producing neurons in the hypothalamus. It is tightly associated with a specific human leukocyte antigen (HLA)-allele: HLA - DQB1 * 06 : 02 . Based on this, an autoimmune process has been hypothesized. A functional HLA - DQ molecule consists of a DQ α and a DQ β chain. HLA - DQB1 * 06 : 02 ( DQ β) has a strong preference for binding to HLA - DQA1 * 01 : 02 ( DQ α), and together they form the functional DQ0602 dimer. A dosage effect would be expected if the HLA - DQ0602 dimer itself is directly involved in the aetiology. An increased expression of the HLA - DQ0602 dimer is expected in individuals homozygous for HLA - DQB1 * 06 : 02 - DQA1 * 01 : 02 , but is also hypothesized in individuals heterozygous for HLA - DQB1 * 06 : 02 and homozygous for HLA - DQA1 * 01 : 02 . To study the impact of the expression of the HLA - DQ0602 dimer on narcolepsy susceptibility, 248 Dutch narcolepsy patients and 1272 Dutch control subjects, all of them positive for DQB1 * 06 : 02 (heterozygous and homozygous), were HLA-genotyped with attention not only to DQB1 but also to DQA1 * 01 : 02. DQB1 * 06 : 02 - DQA1 * 01 : 02 homozygosity was significantly more often seen in patients compared to controls (O.R. 2.29) confirming previous observations. More importantly, a significantly higher prevalence of homozygosity for DQA1 * 01 : 02 was found in HLA - DQB1 * 06 : 02 heterozygous patients compared to controls (O.R. 2.37, p  < 0.001). The latter finding clearly supports a direct role of the HLA - DQ molecule in the development of disease.

• Publisher: Springer Berlin Heidelberg,Springer Nature B.V
• Subject: Alleles / Allergology / Antigens / Autoimmune Diseases - genetics / Autoimmune Diseases - immunology / Biomedical and Life Sciences / Biomedicine / Cell Biology / Drug dosages / Etiology / Gene Function / Genetic Predisposition to Disease / Haplotypes / Heterozygote / Histocompatibility Testing / HLA-DQ beta-Chains - chemistry / HLA-DQ beta-Chains - genetics / HLA-DQ beta-Chains - immunology / Homozygosity / Homozygote / Human Genetics / Humans / Hypothalamus / Immunology / Intracellular Signaling Peptides and Proteins - immunology / Intracellular Signaling Peptides and Proteins - secretion / Leukocytes / Narcolepsy - etiology / Narcolepsy - genetics / Narcolepsy - immunology / Neurons - immunology / Neurons - secretion / Neuropeptides - immunology / Neuropeptides - secretion / Orexins / Original Paper / Sleep disorders