Asset Details
Do you wish to reserve the book?
Bright photoactivatable fluorophores for single-molecule imaging
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Bright photoactivatable fluorophores for single-molecule imaging
Do you wish to request the book?
Bright photoactivatable fluorophores for single-molecule imaging
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Bright photoactivatable fluorophores for single-molecule imaging
2016
Overview
Photoactivatable derivatives of the bright and photostable Janelia Fluor dyes enable improved multicolor single-particle tracking and facile localization microscopy in cells.
Small-molecule fluorophores are important tools for advanced imaging experiments. We previously reported a general method to improve small, cell-permeable fluorophores which resulted in the azetidine-containing 'Janelia Fluor' (JF) dyes. Here, we refine and extend the utility of these dyes by synthesizing photoactivatable derivatives that are compatible with live-cell labeling strategies. Once activated, these derived compounds retain the superior brightness and photostability of the JF dyes, enabling improved single-particle tracking and facile localization microscopy experiments.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
/ Animals
/ Biomedical Engineering/Biotechnology
/ Dyes
/ Fluorescent Dyes - chemical synthesis
/ Fluorescent Dyes - chemistry
/ Fluorescent Dyes - metabolism
/ Fluorescent Dyes - radiation effects
/ Humans
/ Ligands
/ Light
/ Mice
/ Molecular diagnostic techniques
/ Recombinant Fusion Proteins - metabolism
/ Single Molecule Imaging - methods
/ Small Molecule Libraries - chemical synthesis
/ Small Molecule Libraries - chemistry
/ Small Molecule Libraries - metabolism
/ Small Molecule Libraries - radiation effects
