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Widespread Translational Inhibition by Plant miRNAs and siRNAs
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Widespread Translational Inhibition by Plant miRNAs and siRNAs
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Journal Article
Widespread Translational Inhibition by Plant miRNAs and siRNAs
2008
Overview
High complementarity between plant microRNAs (miRNAs) and their messenger RNA targets is thought to cause silencing, prevalently by endonucleolytic cleavage. We have isolated Arabidopsis mutants defective in miRNA action. Their analysis provides evidence that plant miRNA-guided silencing has a widespread translational inhibitory component that is genetically separable from endonucleolytic cleavage. We further show that the same is true of silencing mediated by small interfering RNA (siRNA) populations. Translational repression is effected in part by the ARGONAUTE proteins AGO1 and AGO10. It also requires the activity of the microtubule-severing enzyme katanin, implicating cytoskeleton dynamics in miRNA action, as recently suggested from animal studies. Also as in animals, the decapping component VARICOSE (VCS)/Ge-1 is required for translational repression by miRNAs, which suggests that the underlying mechanisms in the two kingdoms are related.
Publisher
American Association for the Advancement of Science,The American Association for the Advancement of Science
Subject
Adenosine Triphosphatases - genetics
/ Adenosine Triphosphatases - physiology
/ animals
/ Arabidopsis Proteins - genetics
/ Arabidopsis Proteins - physiology
/ Biological and medical sciences
/ Botany
/ Enzymes
/ Fundamental and applied biological sciences. Psychology
/ Gene Expression Regulation, Plant
/ Green Fluorescent Proteins - genetics
/ Katanin
/ MicroRNA
/ Molecular and cellular biology
/ mutants
/ Mutation
/ proteins
/ Reverse transcriptase polymerase chain reaction
/ RNA
/ RNA Caps
