Journal Article

A Synbiotic Combining Chitin–Glucan and Lactobacillus acidophilus NCFM Induces a Colonic Molecular Signature Soothing Intestinal Pain and Inflammation in an Animal Model of IBS

Ouwehand, Arthur C., Modica, Salvatore, Capirchio, Lena, Maquet, Véronique, Rousseaux, Christel, Dubuquoy, Caroline, Desreumaux, Pierre, Louis, Edouard, Tack, Jan

2024

Overview

Chitin–glucan (CG) is a new generation of prebiotic. Lactobacillus acidophilus NCFM® (NCFM) is a probiotic with the ability to decrease abdominal pain. We evaluate the functional and molecular gastrointestinal responses to a synbiotic administration combining CG and NCFM in a rat model of long-lasting colon hypersensitivity. The intracolonic pressure was assessed during the 9-week experiment in animals receiving CG in association or not with NCFM and compared to that in Lacticaseibacillus paracasei Lpc-37®-treated animals and control rats receiving tap water. The effects of the synbiotic were evaluated using the Wallace score, the quantification of colon myeloperoxidase (MPO) and the master genes driving analgesia and inflammation. CG 1.5 alone and NCFM 109 colony forming units (CFU) alone similarly decreased the visceral pain sensitivity. Lpc-37 had no significant effect. The best profile of pain perception inhibition was obtained with the combination of CG 1.5 g and NCFM 109 CFU, confirming a synbiotic property. This synbiotic treatment significantly reduced macroscopic colonic lesions and MPO concentrations, and induced master genes involved in analgesia (CB1, CB2, MOR, PPARα), with a downregulation of inflammatory cytokines (IL-1β, TNFα) and an induction of IL-10 and PPARγ. In conclusion, CG 1.5 g + NCFM 109 CFU significantly decreased visceral pain perception and intestinal inflammation through the regulation of master genes.

• Publisher: MDPI AG,Multidisciplinary Digital Publishing Institute (MDPI),MDPI
• Subject: abdominal pain / analgesia / Analgesics / Animals / Care and treatment / Chitin / Chitin - pharmacology / chitin-glucan / Colon - metabolism / Colon - pathology / Colon/metabolism/pathology / Cytokines / Cytokines - metabolism / Disease Models, Animal / EC 1.11.1.7 (Peroxidase) / Food / Gastroenterology & hepatology / Gastroentérologie & hépatologie / Glucans / Glucans - pharmacology / Health aspects / Human health sciences / Inflammation / Irritable bowel syndrome / Irritable Bowel Syndrome - metabolism / Lactobacillus acidophilus / Lactobacillus acidophilus NCFM / Male / Microbiota / Molecular Biology / Molekylärbiologi / Oral administration / Pain / Pathophysiology / Peroxidase - metabolism / Polysaccharides / prebiotic / Prebiotics / probiotic / Probiotics / Probiotics - administration & dosage / Rats / Rats, Wistar / Rodents / Sciences de la santé humaine / synbiotic / Synbiotics - administration & dosage