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Differences in skin test reactions to official and defined antigens in guinea pigs exposed to non-tuberculous and tuberculous bacteria
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Differences in skin test reactions to official and defined antigens in guinea pigs exposed to non-tuberculous and tuberculous bacteria
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Journal Article
Differences in skin test reactions to official and defined antigens in guinea pigs exposed to non-tuberculous and tuberculous bacteria
2023
Overview
The single and comparative intradermal tuberculin tests (SITT and CITT) are official in vivo tests for bovine tuberculosis (TB) diagnosis using bovine and avian purified protein derivatives (PPD-B and PPD-A). Infection with bacteria other than
Mycobacterium tuberculosis
complex (MTC) can result in nonspecific reactions to these tests. We evaluated the performance of the skin test with PPDs and new defined antigens in the guinea pig model. A standard dose (SD) of
Rhodococcus equi
,
Nocardia
sp.,
M
.
nonchromogenicum
,
M
.
monacense
,
M
.
intracellulare
,
M
.
avium
subsp.
paratuberculosis
,
M
.
avium
subsp.
avium
,
M
.
avium
subsp.
hominissuis
,
M
.
scrofulaceum
,
M. persicum
,
M
.
microti
,
M
.
caprae
and
M
.
bovis
, and a higher dose (HD) of
M
.
nonchromogenicum
,
M
.
monacense
,
M
.
intracellulare
,
M
.
avium
subsp.
paratuberculosis
were tested using PPD-B, PPD-A, P22, ESAT-6-CFP-10-Rv3615c peptide cocktail long (PCL) and fusion protein (FP). The SD of
R
.
equi
,
Nocardia
sp.,
M
.
nonchromogenicum
,
M
.
monacense
,
M
.
intracellulare
and
M
.
avium
subsp.
paratuberculosis
did not cause any reactions. The HD of
M
.
nonchromogenicum
,
M
.
monacense
,
M
.
intracellulare
, and
M
.
avium
subsp.
paratuberculosis
and the SD of
M
.
avium
subsp.
hominissuis
,
M
.
scrofulaceum
and
M
.
persicum
, caused nonspecific reactions (SIT). A CITT interpretation would have considered
M
.
avium
complex and
M
.
scrofulaceum
groups negative, but not all individuals from
M
.
nonchromogenicum
HD,
M
.
monacense
HD and
M
.
persicum
SD groups. Only animals exposed to
M. bovis
and
M. caprae
reacted to PCL and FP. These results support the advantage of complementing or replacing PPD-B to improve specificity without losing sensitivity.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
