Journal Article

Isolation and characterization of centroacinar/terminal ductal progenitor cells in adult mouse pancreas

Liss, Andrew S, Jensen, Jan, Scott, Sherri-Gae, Thayer, Sarah P, Rovira, Meritxell, Leach, Steven D

2010

Overview

The question of whether dedicated progenitor cells exist in adult vertebrate pancreas remains controversial. Centroacinar cells and terminal duct (CA/TD) cells lie at the junction between peripheral acinar cells and the adjacent ductal epithelium, and are frequently included among cell types proposed as candidate pancreatic progenitors. However these cells have not previously been isolated in a manner that allows formal assessment of their progenitor capacities. We have found that a subset of adult CA/TD cells are characterized by high levels of ALDH1 enzymatic activity, related to high-level expression of both Aldh1a1 and Aldh1a7. This allows their isolation by FACS using a fluorogenic ALDH1 substrate. FACS-isolated CA/TD cells are relatively depleted of transcripts associated with differentiated pancreatic cell types. In contrast, they are markedly enriched for transcripts encoding Sca1, Sdf1, c-Met, Nestin, and Sox9, markers previously associated with progenitor populations in embryonic pancreas and other tissues. FACS-sorted CA/TD cells are uniquely able to form self-renewing \"pancreatospheres\" in suspension culture, even when plated at clonal density. These spheres display a capacity for spontaneous endocrine and exocrine differentiation, as well as glucose-responsive insulin secretion. In addition, when injected into cultured embryonic dorsal pancreatic buds, these adult cells display a unique capacity to contribute to both the embryonic endocrine and exocrine lineages. Finally, these cells demonstrate dramatic expansion in the setting of chronic epithelial injury. These findings suggest that CA/TD cells are indeed capable of progenitor function and may contribute to the maintenance of tissue homeostasis in adult mouse pancreas.

• Publisher: National Academy of Sciences,National Acad Sciences
• Subject: Acinar cells / Adult stem cells / adults / Aldehyde Dehydrogenase - genetics / Aldehyde Dehydrogenase - metabolism / Animals / Biological Sciences / Biomarkers - metabolism / Cell Lineage / Cell Separation - methods / Cells / Enzymatic activity / enzyme activity / Enzyme kinetics / Epithelial cells / Epithelial Cells - cytology / Epithelial Cells - pathology / epithelium / Exocrine cells / Flow Cytometry - methods / Fluorescent Dyes - metabolism / Gene Expression / homeostasis / Humans / insulin secretion / Isoenzymes - genetics / Isoenzymes - metabolism / Mesenchymal stem cells / Mice / Multipotent stem cells / Pancreas / Pancreas - cytology / Pancreas - enzymology / Pancreas - physiology / Pancreatic cells / Progenitor cells / Retinal Dehydrogenase / Rodents / Stem cells / Stem Cells - cytology / Stem Cells - physiology / Tissues