Journal Article

Aquaculture changes the profile of antibiotic resistance and mobile genetic element associated genes in Baltic Sea sediments

Stedtfeld, Robert D., Lyra, Christina, Pärnänen, Katariina, Johnson, Timothy A., Karkman, Antti, Muziasari, Windi I., Tiedje, James M., Virta, Marko, Tamminen, Manu

2016

Overview

Antibiotics are commonly used in aquaculture and they can change the environmental resistome by increasing antibiotic resistance genes (ARGs). Sediment samples were collected from two fish farms located in the Northern Baltic Sea, Finland, and from a site outside the farms (control). The sediment resistome was assessed by using a highly parallel qPCR array containing 295 primer sets to detect ARGs, mobile genetic elements and the 16S rRNA gene. The fish farm resistomes were enriched in transposon and integron associated genes and in ARGs encoding resistance to antibiotics which had been used to treat fish at the farms. Aminoglycoside resistance genes were also enriched in the farm sediments despite the farms not having used aminoglycosides. In contrast, the total relative abundance values of ARGs were higher in the control sediment resistome and they were mainly genes encoding efflux pumps followed by beta-lactam resistance genes, which are found intrinsically in many bacteria. This suggests that there is a natural Baltic sediment resistome. The resistome associated with fish farms can be from native ARGs enriched by antibiotic use at the farms and/or from ARGs and mobile elements that have been introduced by fish farming. Antibiotic resistome associated with fish farms can be from native antibiotic resistance genes (ARGs) enriched by antibiotic use at the farms and/or from ARGs and mobile elements that have been introduced by fish farming. Graphical Abstract Figure. Antibiotic resistome associated with fish farms can be from native antibiotic resistance genes (ARGs) enriched by antibiotic use at the farms and/or from ARGs and mobile elements that have been introduced by fish farming.

• Publisher: Oxford University Press
• Subject: aminoglycosides / Aminoglycosides - pharmacology / Animals / Anti-Bacterial Agents - pharmacology / antibiotic resistance / antibiotic resistance genes / antibiotics / Aquaculture / Aquaculture - methods / bacteria / Bacteria - drug effects / Bacteria - genetics / Baltic Sea / beta-Lactam Resistance - genetics / beta-lactams / beta-Lactams - pharmacology / Drug resistance in microorganisms / Drug Resistance, Bacterial - genetics / Environmental aspects / Finland / fish / fish farms / Fish-culture / Fisheries / Genes, Bacterial / Genetic aspects / Geologic Sediments - microbiology / Integrons / Interspersed Repetitive Sequences - genetics / Marine sediments / Microbial Sensitivity Tests / Oceans and Seas / oligodeoxyribonucleotides / Polymerase Chain Reaction / quantitative polymerase chain reaction / ribosomal RNA / RNA, Ribosomal, 16S - genetics / sediments / transporters / transposons