Asset Details
Do you wish to reserve the book?
Dynamic maintenance of asymmetric meiotic spindle position through Arp2/3-complex-driven cytoplasmic streaming in mouse oocytes
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Dynamic maintenance of asymmetric meiotic spindle position through Arp2/3-complex-driven cytoplasmic streaming in mouse oocytes
Do you wish to request the book?
Dynamic maintenance of asymmetric meiotic spindle position through Arp2/3-complex-driven cytoplasmic streaming in mouse oocytes
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Dynamic maintenance of asymmetric meiotic spindle position through Arp2/3-complex-driven cytoplasmic streaming in mouse oocytes
2011
Overview
Mammalian oocyte maturation involves two asymmetric meiotic divisions that require the positioning of the meiotic spindle near the cortical area from which the extrusion of the polar bodies occurs. Li and colleagues show that the nucleating activity of the Arp2/3 complex, localized at the cortical actin cap, induces actin-filament flow away from the complex, creating a cytoplasmic streaming that pushes the spindle towards the cortex.
Mature mammalian oocytes are poised for completing meiosis II (MII) on fertilization by positioning the spindle close to an actomyosin-rich cortical cap
1
,
2
,
3
. Here, we show that the Arp2/3 complex localizes to the cortical cap in a Ran-GTPase-dependent manner and nucleates actin filaments in the cortical cap and a cytoplasmic actin network. Inhibition of Arp2/3 activity leads to rapid dissociation of the spindle from the cortex. Live-cell imaging and spatiotemporal image correlation spectroscopy analysis reveal that actin filaments flow continuously away from the Arp2/3-rich cortex, driving a cytoplasmic streaming expected to exert a net pushing force on the spindle towards the cortex. Arp2/3 inhibition not only diminishes this actin flow and cytoplasmic streaming but also enables a reverse streaming driven by myosin-II-based cortical contraction, moving the spindle away from the cortex. Thus, the asymmetric MII spindle position is dynamically maintained as a result of balanced forces governed by the Arp2/3 complex.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ Actin Cytoskeleton - drug effects
/ Actin Cytoskeleton - metabolism
/ Actin-Related Protein 2 - genetics
/ Actin-Related Protein 2 - metabolism
/ Actin-Related Protein 2-3 Complex - antagonists & inhibitors
/ Actin-Related Protein 2-3 Complex - genetics
/ Actin-Related Protein 2-3 Complex - metabolism
/ Actin-Related Protein 3 - genetics
/ Actin-Related Protein 3 - metabolism
/ Animals
/ Biomedical and Life Sciences
/ Cytoplasmic Streaming - drug effects
/ Female
/ Heterocyclic Compounds, 4 or More Rings - pharmacology
/ letter
/ Mammals
/ Mice
/ Oocytes
/ Proteins
/ ran GTP-Binding Protein - metabolism
/ Recombinant Fusion Proteins - metabolism
/ Spindle Apparatus - drug effects
