Asset Details
Do you wish to reserve the book?
Mycobacterial growth inhibition is associated with trained innate immunity
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Mycobacterial growth inhibition is associated with trained innate immunity
Do you wish to request the book?
Mycobacterial growth inhibition is associated with trained innate immunity
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Mycobacterial growth inhibition is associated with trained innate immunity
2018
Overview
The lack of defined correlates of protection hampers development of vaccines against tuberculosis (TB). In vitro mycobacterial outgrowth assays are thought to better capture the complexity of the human host/Mycobacterium tuberculosis (Mtb) interaction. Here, we used a mycobacterial growth inhibition assay (MGIA) based on peripheral blood mononuclear cells to investigate the capacity to control outgrowth of bacille Calmette-Guérin (BCG). Interestingly, strong control of BCG outgrowth was observed almost exclusively in individuals with recent exposure to Mtb, but not in (long-term) latent TB infection, and only modestly in BCG vaccinees. Mechanistically, control of mycobacterial outgrowth strongly correlated with the presence of a CD14dim monocyte population, but also required the presence of T cells. The nonclassical monocytes produced CXCL10, and CXCR3 receptor blockade inhibited the capacity to control BCG outgrowth. Expression of CXCR3 splice variants was altered in recently Mtb-exposed individuals. Cytokines previously associated with trained immunity were detected in MGIA supernatants, and CXCL9, CXCL10, and CXCL11 represent new markers of trained immunity. These data indicate that CXCR3 ligands are associated with trained immunity and are critical factors in controlling mycobacterial outgrowth. In conclusion, control of mycobacterial outgrowth early after exposure to Mtb is the result of trained immunity mediated by a CXCL10-producing nonclassical CD14dim monocyte subset.
Publisher
American Society for Clinical Investigation
Subject
/ Bacillus Calmette-Guerin vaccine
/ BCG
/ Chemokines, CXC - immunology
/ Female
/ Host-Pathogen Interactions - immunology
/ Humans
/ Latent Tuberculosis - immunology
/ Latent Tuberculosis - pathology
/ Leukocytes, Mononuclear - immunology
/ Leukocytes, Mononuclear - microbiology
/ Leukocytes, Mononuclear - pathology
/ Male
/ Mycobacterium tuberculosis - growth & development
/ Mycobacterium tuberculosis - immunology
/ Peripheral blood mononuclear cells
