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Identification of two major autoantigens negatively regulating endothelial activation in Takayasu arteritis
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Identification of two major autoantigens negatively regulating endothelial activation in Takayasu arteritis
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Journal Article
Identification of two major autoantigens negatively regulating endothelial activation in Takayasu arteritis
2020
Overview
The presence of antiendothelial cell antibodies (AECAs) has been documented in Takayasu arteritis (TAK), a chronic granulomatous vasculitis. Here, we identify cell-surface autoantigens using an expression cloning system. A cDNA library of endothelial cells is retrovirally transfected into a rat myeloma cell line from which AECA-positive clones are sorted with flow cytometry. Four distinct AECA-positive clones are isolated, and endothelial protein C receptor (EPCR) and scavenger receptor class B type 1 (SR-BI) are identified as endothelial autoantigens. Autoantibodies against EPCR and SR-BI are detected in 34.6% and 36.5% of cases, respectively, with minimal overlap (3.8%). Autoantibodies against EPCR are also detected in ulcerative colitis, the frequent comorbidity of TAK. In mechanistic studies, EPCR and SR-BI function as negative regulators of endothelial activation. EPCR has also an effect on human T cells and impair Th17 differentiation. Autoantibodies against EPCR and SR-BI block the functions of their targets, thereby promoting pro-inflammatory phenotype.
Autoantibodies against endothelium have been recognized in Takayasu arteritis (TAK). Here the authors identify endothelial protein C receptor and scavenger receptor class B type 1 as major autoantigens in TAK, and find autoantibodies inhibit the negative regulation of endothelial activation.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
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/ 14
/ 14/34
/ 14/63
/ 38/39
/ 38/61
/ 38/77
/ 42/40
/ 45
/ 45/22
/ 45/29
/ Animals
/ Antigens
/ Autoantibodies - isolation & purification
/ Autoantigens - isolation & purification
/ Cloning
/ Colitis, Ulcerative - immunology
/ Endothelial Cells - immunology
/ Endothelial Protein C Receptor
/ Endothelium, Vascular - metabolism
/ Humanities and Social Sciences
/ Humans
/ Medicine
/ Multiple Myeloma - metabolism
/ Myeloma
/ Proteins
/ Rats
/ Receptors, Endothelin - metabolism
/ Scavenger Receptors, Class B - metabolism
/ Science
/ Takayasu Arteritis - immunology
