Journal Article

Biocompatible nucleus-targeted graphene quantum dots for selective killing of cancer cells via DNA damage

Wu, Zixia, Cao Dayong, Pan Tonghe, Ou Liling, Bao Bijun, Qi Lei, Dai Liming, Ye Zhiqiang, Yu, Chunlei

2021

Overview

Graphene quantum dots (GQDs) are nano-sized graphene slices. With their small size, lamellar and aromatic-ring structure, GQDs tend to enter into the cell nucleus and interfere with DNA activity. Thus, GQD alone is expected to be an anticancer reagent. Herein, we developed GQDs that suppress the growth of tumor by selectively damaging the DNA of cancer cells. The amine-functionalized GQDs were modified with nucleus targeting TAT peptides (TAT-NGs) and further grafted with cancer-cell-targeting folic acid (FA) modified PEG via disulfide linkage (FAPEG-TNGs). The resulting FAPEG-TNGs exhibited good biocompatibility, nucleus uptake, and cancer cell targeting. They adsorb on DNA via the π–π and electrostatic interactions, which induce the DNA damage, the upregulation of the cell apoptosis related proteins, and the suppression of cancer cell growth, ultimately. This work presents a rational design of GQDs that induce the DNA damage to realize high therapeutic performance, leading to a distinct chemotherapy strategy for targeted tumor therapy.Qi et al. develop nucleus targeting graphene quantum dots (GQDs) by modifying amine-functionalised GQDs with nucleus targeting TAT peptides. The resulting functionalised GQDs exhibit good biocompatibility, nucleus uptake, and cancer cell targeting. They can suppress growth of cancer cells by selectively inducing DNA damage.

• Publisher: Nature Publishing Group
• Subject: Apoptosis / Biocompatibility / Biology / Cancer / Chemotherapy / Deoxyribonucleic acid / DNA / DNA damage / Electrostatic properties / Folic acid / Graphene / Peptides / Quantum dots