Journal Article

Expression of the Arabidopsis WRINKLED 1 transcription factor leads to higher accumulation of palmitate in soybean seed

Changa, Taity, Nguyen, Hanh, Vogel, Pamela A., Clemente, Tom Elmo, Cahoon, Edgar B., Hou, Lili, Park, Hyunwoo, Ciftci, Ozan N., Bayon de Noyer, Shen, Wang, Tong, Khang, Hoang Le

2019

Overview

Summary Soybean (Glycine max [L.] Merr.) is a commodity crop highly valued for its protein and oil content. The high percentage of polyunsaturated fatty acids in soybean oil results in low oxidative stability, which is a key parameter for usage in baking, high temperature frying applications, and affects shelf life of packaged products containing soybean oil. Introduction of a seed‐specific expression cassette carrying the Arabidopsis transcription factor WRINKLED1 (AtWRI1) into soybean, led to seed oil with levels of palmitate up to approximately 20%. Stacking of the AtWRI1 transgenic allele with a transgenic locus harbouring the mangosteen steroyl‐ACP thioesterase (GmFatA) resulted in oil with total saturates up to 30%. The creation of a triple stack in soybean, wherein the AtWRI1 and GmFatA alleles were combined with a FAD2‐1 silencing allele led to the synthesis of an oil with 28% saturates and approximately 60% oleate. Constructs were then assembled that carry a dual FAD2‐1 silencing element/GmFatA expression cassette, alone or combined with an AtWRI1 cassette. These plasmids are designated pPTN1289 and pPTN1301, respectively. Transgenic events carrying the T‐DNA of pPTN1289 displayed an oil with stearate levels between 18% and 25%, and oleate in the upper 60%, with reduced palmitate (<5%). While soybean events harboring transgenic alleles of pPTN1301 had similar levels of stearic and oleate levels as that of the pPTRN1289 events, but with levels of palmitate closer to wild type. The modified fatty acid composition results in an oil with higher oxidative stability, and functionality attributes for end use in baking applications.

• Publisher: John Wiley & Sons, Inc,John Wiley and Sons Inc
• Subject: Alleles / Arabidopsis / Arabidopsis Proteins - genetics / Arabidopsis thaliana / Baking / Biodiesel fuels / Biosynthesis / biotechnology / Edible oils / Embryos / Enzymes / Fatty acid composition / Fatty acids / Frying / Gene silencing / Genes / Genetic engineering / genetically modified organisms / Glycine max / Glycine max - genetics / Glycine max - metabolism / high solids soybean oil / High temperature / Hydrogenation / lipid content / loci / mangosteens / Oils & fats / oleic acid / oxidative stability / palmitates / Palmitates - analysis / Palmitic acid / Palmitoyl-(protein) hydrolase / Plant Oils - chemistry / Plants, Genetically Modified - metabolism / Plasmids / Polyunsaturated fatty acids / Proteins / Saturated fatty acids / Seeds / Seeds - chemistry / Shelf life / Software / Soybean / Soybean oil / Soybeans / Stability / Statistical analysis / stearic acid / temperature / Thioesterase / Transcription factors / Transcription Factors - genetics / Unsaturated fatty acids / Variance analysis / Vegetable oils