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Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells
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Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells
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Journal Article
Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells
2009
Overview
Ectopic expression of transcription factors can reprogram somatic cells to a pluripotent state. However, most of the studies used skin fibroblasts as the starting population for reprogramming, which usually take weeks for expansion from a single biopsy. We show here that induced pluripotent stem (iPS) cells can be generated from adult human adipose stem cells (hASCs) freshly isolated from patients. Furthermore, iPS cells can be readily derived from adult hASCs in a feeder-free condition, thereby eliminating potential variability caused by using feeder cells. hASCs can be safely and readily isolated from adult humans in large quantities without extended time for expansion, are easy to maintain in culture, and therefore represent an ideal autologous source of cells for generating individual-specific iPS cells.
Publisher
National Academy of Sciences,National Acad Sciences
Subject
/ Adult
/ Adult Stem Cells - immunology
/ Adult Stem Cells - metabolism
/ Adults
/ Aged
/ Alkaline Phosphatase - metabolism
/ Antigens, Surface - metabolism
/ biopsy
/ Cell Culture Techniques - methods
/ Cell Dedifferentiation - genetics
/ Cell Dedifferentiation - immunology
/ Cell Dedifferentiation - physiology
/ Humans
/ Induced pluripotent stem cells
/ patients
/ Pluripotent Stem Cells - cytology
/ Pluripotent Stem Cells - immunology
