Journal Article

A multifunctional platform with single-NIR-laser-triggered photothermal and NO release for synergistic therapy against multidrug-resistant Gram-negative bacteria and their biofilms

Li, Haisheng, He, Weifeng, Tan, Jianglin, Zhou, Junyi, Wang, He, Li, Lanlan, Zhang, Jianxiang, Dong, Wenjing, Zhao, Baohua, Luo, Gaoxing, Qian, Wei, Cheng, Shaowen

2020

Overview

Background Infectious diseases caused by multidrug-resistant (MDR) bacteria, especially MDR Gram-negative strains, have become a global public health challenge. Multifunctional nanomaterials for controlling MDR bacterial infections via eradication of planktonic bacteria and their biofilms are of great interest. Results In this study, we developed a multifunctional platform (TG-NO-B) with single NIR laser-triggered PTT and NO release for synergistic therapy against MDR Gram-negative bacteria and their biofilms. When located at the infected sites, TG-NO-B was able to selectively bind to the surfaces of Gram-negative bacterial cells and their biofilm matrix through covalent coupling between the BA groups of TG-NO-B and the bacterial LPS units, which could greatly improve the antibacterial efficiency, and reduce side damages to ambient normal tissues. Upon single NIR laser irradiation, TG-NO-B could generate hyperthermia and simultaneously release NO, which would synergistically disrupt bacterial cell membrane, further cause leakage and damage of intracellular components, and finally induce bacteria death. On one hand, the combination of NO and PTT could largely improve the antibacterial efficiency. On the other hand, the bacterial cell membrane damage could improve the permeability and sensitivity to heat, decrease the photothermal temperature and avoid damages caused by high temperature. Moreover, TG-NO-B could be effectively utilized for synergistic therapy against the in vivo infections of MDR Gram-negative bacteria and their biofilms and accelerate wound healing as well as exhibit excellent biocompatibility both in vitro and in vivo. Conclusions Our study demonstrates that TG-NO-B can be considered as a promising alternative for treating infections caused by MDR Gram-negative bacteria and their biofilms.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Animals / Antiinfectives and antibacterials / Bacteria / Bacterial diseases / Bacterial infections / Biocompatibility / Biocompatible Materials - chemistry / Biocompatible Materials - metabolism / Biocompatible Materials - pharmacology / Biofilms / Biofilms - drug effects / Biofilms - radiation effects / Biotechnology / Cell membranes / Cell Survival - drug effects / Chemistry / Chemistry and Materials Science / Drug resistance / Drug Resistance, Multiple, Bacterial - drug effects / Drug Resistance, Multiple, Bacterial - radiation effects / Fever / Gram-negative bacteria / Gram-Negative Bacteria - physiology / Gram-Negative Bacterial Infections - pathology / Gram-Negative Bacterial Infections - therapy / Gram-Negative Bacterial Infections - veterinary / Graphene / Graphite - chemistry / Health aspects / Hemolysis - drug effects / High temperature / Hyperthermia / In vivo methods and tests / Infection / Infection control / Infections / Infectious diseases / Infrared Rays / Irradiation / Lipopolysaccharides / Membrane permeability / Methicillin / Mice / Mice, Inbred BALB C / Microbial drug resistance / Microbial Sensitivity Tests / Molecular Medicine / Multidrug resistance / Multidrug resistant organisms / Multidrug-resistant Gram-negative bacteria / Nanomaterials / Nanostructures - chemistry / Nanostructures - toxicity / Nanotechnology / NIH 3T3 Cells / Nitrogen Oxides - metabolism / NO release / Permeability / Phototherapy / Photothermal / Public health / Radiation damage / Scientific equipment industry / Single-NIR-laser-triggered / Staphylococcus infections / Synergistic / Temperature / Therapy / Tissue Distribution / Wound healing / Wound Healing - drug effects / Wound Healing - radiation effects