Journal Article

Protective immunity against Trichinella spiralis in mice elicited by oral vaccination with attenuated Salmonella-delivered TsSP1.2 DNA

Qi, Xin, Guo, Kai Xia, Han, Yue, Lei, Jun Jun, Cheng, Xiang Chao, Jiang, Peng, Li, Jie Feng, Wang, Zhong Quan, Yan, Shu Wei, Yu, Chuan, Cui, Jing

2018

Overview

Trichinellosis is a worldwide important food-borne zoonosis caused mainly by ingesting raw or undercooked pork infected with Trichinella spiralis larvae. The development of vaccine is needed for preventing swine from Trichinella infection to ensure pork safety. Previous studies showed that T. spiralis serine protease 1.2 (TsSP1.2) is a vaccine candidate against Trichinella infection. In this study, the complete TsSP1.2 cDNA sequences were cloned into pcDNA3.1, and the rTsSP1.2 DNA was transformed into attenuated Salmonella typhimurium strain ΔcyaSL1344. Oral vaccination of mice with Salmonella -delivered rTsSP1.2 DNA vaccine induced an obvious intestinal mucosal IgA response and a systemic Th1/Th2 immune response; the vaccinated mice showed a 33.45% reduction of intestinal adult worms and 71.84% reduction of muscle larvae after T. spiralis larval challenge. The protection might be due to the rTsSP1.2-induced production of specific anti-TsSP1.2 sIgA, IgG, IgG1/IgG2a, and secretion of IFN-γ, IL-4 and IL-10, which protected intestinal mucosa from the parasite invasion, inhibited worm development and reduced female fecundity. The results indicate that the attenuated Salmonella -delivered rTsSP1.2 DNA vaccine offers a prospective strategy for the prevention and control of animal Trichinella infection.

• Publisher: BioMed Central,BioMed Central Ltd,BMC
• Subject: Administration, Oral / adults / Animal vaccination / Animals / complementary DNA / fecundity / Female / females / Health aspects / Helminth Proteins - immunology / Immune response / Immunity, Mucosal / immunoglobulin A / immunoglobulin G / Immunology / interferon-gamma / interleukin-10 / interleukin-4 / intestinal mucosa / Life Sciences / Medicine / Medicine & Public Health / Mice / Mice, Inbred BALB C / Microbiology / Microorganisms, Genetically-Modified - genetics / muscle larvae / nucleotide sequences / oral vaccination / parasites / pork / Prevention / recombinant vaccines / Research Article / Salmonella Typhimurium / Salmonella typhimurium - genetics / secretion / Serine Proteases - immunology / serine proteinases / swine / Swine diseases / Trichina worm / Trichinella spiralis / Trichinella spiralis - immunology / trichinosis / Vaccination / vaccine development / Vaccines, Attenuated - administration & dosage / Vaccines, DNA - administration & dosage / veterinary medicine / Veterinary Medicine/Veterinary Science / Veterinary research / Virology / zoonoses