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Isolation and characterization of a roseophage representing a novel genus in the N4-like Rhodovirinae subfamily distributed in estuarine waters
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Isolation and characterization of a roseophage representing a novel genus in the N4-like Rhodovirinae subfamily distributed in estuarine waters
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Isolation and characterization of a roseophage representing a novel genus in the N4-like Rhodovirinae subfamily distributed in estuarine waters
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Journal Article
Isolation and characterization of a roseophage representing a novel genus in the N4-like Rhodovirinae subfamily distributed in estuarine waters
2025
Overview
Background
Roseobacteraceae
, often referred to as the marine roseobacter clade (MRC), are pivotal constituents of bacterial communities in coastal and pelagic marine environments. During the past two decades, 75 roseophages that infect various
Roseobacteraceae
lineages have been isolated. The N4-like roseophage clade, which encompasses 15 members, represents the largest clade among these roseophages. N4-like phages form a monophyletic group, classified as family
Schitoviridae
. And all N4-like roseophages form a unique clade within
Schitoviridae
and has been classified as the
Rhodovirinae
subfamily.
Results
In this study, we isolated a novel roseophage, vB_DshP-R7L, that infects
Dinoroseobacter shibae
DFL12 from Xiamen Bay in the East China Sea. Conserved genes of
Schitoviridae
have been identified in the genome of vB_DshP-R7L, and following phylogenetic analysis suggests that the newly isolated phage is a member of the
Rhodovirinae
subfamily and represents the sole member of a novel genus,
Gonggongvirus
. The genome of vB_DshP-R7L harbors six auxiliary metabolic genes (AMGs), most of which potentially enhance DNA de novo synthesis. Additionally, a gene encoding ribosomal protein was identified. Comparative genomic analysis of AMG content among
Rhodovirinae
indicates a distinct evolutionary history characterized by independent ancient horizontal gene transfer events. Read-mapping analysis reveals the prevalence of vB_DshP-R7L and other
Rhodovirinae
roseophages in estuarine waters.
Conclusions
Our work illustrates the genomic features of a novel roseophage clade among the subfamily
Rhodovirinae.
The AMG content of vB_DshP-R7L is under severe purification selection, which reveals their possible ecological importance. We also demonstrated that vB_DshP-R7L and other
Rhodovirinae
roseophages are only detected in estuaries. Our isolation and characterization of this novel phage expands the understanding of the phylogeny, gene transfer history, and biogeography of
Rhodovirinae
infecting marine
Roseobacteraceae.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
