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DNA methylation loss in late-replicating domains is linked to mitotic cell division
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DNA methylation loss in late-replicating domains is linked to mitotic cell division
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Journal Article
DNA methylation loss in late-replicating domains is linked to mitotic cell division
2018
Overview
DNA methylation loss occurs frequently in cancer genomes, primarily within lamina-associated, late-replicating regions termed partially methylated domains (PMDs). We profiled 39 diverse primary tumors and 8 matched adjacent tissues using whole-genome bisulfite sequencing (WGBS) and analyzed them alongside 343 additional human and 206 mouse WGBS datasets. We identified a local CpG sequence context associated with preferential hypomethylation in PMDs. Analysis of CpGs in this context (‘solo-WCGWs’) identified previously undetected PMD hypomethylation in almost all healthy tissue types. PMD hypomethylation increased with age, beginning during fetal development, and appeared to track the accumulation of cell divisions. In cancer, PMD hypomethylation depth correlated with somatic mutation density and cell cycle gene expression, consistent with its reflection of mitotic history and suggesting its application as a mitotic clock. We propose that late replication leads to lifelong progressive methylation loss, which acts as a biomarker for cellular aging and which may contribute to oncogenesis.
Whole-genome DNA methylation profiling and analysis of normal tissues from both human and mouse reveal that hypomethylation within partially methylated, late-replicating domains depends on sequence context, starts early in development, accumulates with cell divisions and progresses with organismal aging.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
/ 45/23
/ 45/61
/ 64/60
/ Age
/ Aging
/ Analysis
/ Animal Genetics and Genomics
/ Animals
/ B cells
/ Biomedical and Life Sciences
/ Cancer
/ DNA
/ Domains
/ Embryonic Development - genetics
/ Female
/ Fetuses
/ Genes
/ Genomes
/ Genomics
/ Humans
/ Male
/ Mice
/ Mutation
/ Sulfites
/ T cells
/ Tumors
