Journal Article

Ambient long-term exposure to organophosphorus pesticides and the human gut microbiome: an observational study

Paul, Kimberly, Cockburn, Myles G., Zhang, Keren, Bronstein, Jeff M., Jacobs, Jonathan P., del Rosario, Irish, Ritz, Beate

2024

Overview

Background Organophosphorus pesticides (OP) have been associated with various human health conditions. Animal experiments and in-vitro models suggested that OP may also affect the gut microbiota. We examined associations between ambient chronic exposure to OP and gut microbial changes in humans. Methods We recruited 190 participants from a community-based epidemiologic study of Parkinson’s disease living in a region known for heavy agricultural pesticide use in California. Of these, 61% of participants had Parkinson’s disease and their mean age was 72 years. Microbiome and predicted metagenome data were generated by 16S rRNA gene sequencing of fecal samples. Ambient long-term OP exposures were assessed using pesticide application records combined with residential addresses in a geographic information system. We examined gut microbiome differences due to OP exposures, specifically differences in microbial diversity based on the Shannon index and Bray–Curtis dissimilarities, and differential taxa abundance and predicted Metacyc pathway expression relying on regression models and adjusting for potential confounders. Results OP exposure was not associated with alpha or beta diversity of the gut microbiome. However, the predicted metagenome was sparser and less evenly expressed among those highly exposed to OP ( p  = 0.04). Additionally, we found that the abundance of two bacterial families, 22 genera, and the predicted expression of 34 Metacyc pathways were associated with long-term OP exposure. These pathways included perturbed processes related to cellular respiration, increased biosynthesis and degradation of compounds related to bacterial wall structure, increased biosynthesis of RNA/DNA precursors, and decreased synthesis of Vitamin B1 and B6. Conclusion In support of previous animal studies and in-vitro findings, our results suggest that ambient chronic OP pesticide exposure alters gut microbiome composition and its predicted metabolism in humans.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Abundance / Aged / Agrochemicals / Analysis / Animal experimentation / Animal models / Animal research / Bacteria / Biodegradation / Biological diversity / Biosynthesis / Brain research / Chronic exposure / Disease / DNA biosynthesis / Dysbiosis / Earth and Environmental Science / Environment / Environmental Health / Epidemiology / Exposure / Feces / Fungicides / Gastrointestinal Microbiome / Gastrointestinal system / Gene sequencing / Genes / Geographic information system / Geographic information systems / Gut microbiome / Gut microbiota / Health aspects / Humans / Insecticides / Intestinal microflora / Medical research / Medicine, Experimental / Metabolism / Metabolites / Microbiomes / Microbiota / Microbiota (Symbiotic organisms) / Microorganisms / Neurodegenerative diseases / Observational studies / Occupational Medicine/Industrial Medicine / Organophosphorus Compounds / Organophosphorus pesticides / Parkinson Disease / Parkinson's disease / Pesticide application / Pesticides / Pesticides - adverse effects / Physiological aspects / Predicted metagenome / Public Health / Regression analysis / Regression models / Remote sensing / Risk factors / RNA / RNA, Ribosomal, 16S - genetics / rRNA 16S / Taxonomy / Toxicity