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Circulating long non-coding RNA GAS5 (growth arrest-specific transcript 5) as a complement marker for the detection of malignant mesothelioma using liquid biopsies
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Circulating long non-coding RNA GAS5 (growth arrest-specific transcript 5) as a complement marker for the detection of malignant mesothelioma using liquid biopsies
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Circulating long non-coding RNA GAS5 (growth arrest-specific transcript 5) as a complement marker for the detection of malignant mesothelioma using liquid biopsies
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Journal Article
Circulating long non-coding RNA GAS5 (growth arrest-specific transcript 5) as a complement marker for the detection of malignant mesothelioma using liquid biopsies
2020
Overview
Background
For the detection of malignant mesothelioma additional markers are needed besides the established panel consisting of calretinin and mesothelin. The aim of this study was the identification and verification of long non-coding RNAs (lncRNAs) as complementing circulating markers.
Methods
Candidate lncRNAs were identified in silico using previously published RNA expression profiles and verified using quantitative PCR (qPCR) in mesothelioma cell lines as well as human plasma samples from mesothelioma patients and asbestos-exposed controls.
Results
GAS5
(growth arrest-specific transcript 5) as a single marker is marked by a low sensitivity of 14%, but the combination of
GAS5
with calretinin and mesothelin increased the panel’s sensitivity from 64 to 73% at a predefined specificity of 97%. Circulating
GAS5
is not affected by pleurectomy before blood collection, age, or smoking status.
Conclusions
GAS5
is verified as an appropriate circulating marker for the supplement of calretinin and mesothelin to detect malignant mesothelioma. Although the sensitivity of
GAS5
is too low for the use as a single marker, the addition of
GAS5
as a third marker improves the performance of the established marker panel. The benefit of
GAS5
for the detection of malignant mesothelioma at early stages needs to be validated in a prospective study.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Biomedical and Life Sciences
/ Biopsy
/ Blood
/ Cancer
/ DNA
/ EDTA
/ Growth
/ Plasma
/ RNA
