Journal Article

Structural basis for enzymatic photocatalysis in chlorophyll biosynthesis

Heyes, Derren J., Sun, Wenli, Cheng, Qi, Johannissen, Linus O., Hay, Sam, Yang, Ji, Hoeven, Robin, Feng, Lingling, Sakuma, Michiyo, Scrutton, Nigel S., Rao, Zihe, Lin, Min, Liu, Huanting, Hardman, Samantha J. O., Leys, David, Levy, Colin W., Zhang, Shaowei, Yu, Xiaolan, Li, Xuemei, Zhou, Aiwu

2019

Overview

The enzyme protochlorophyllide oxidoreductase (POR) catalyses a light-dependent step in chlorophyll biosynthesis that is essential to photosynthesis and, ultimately, all life on Earth 1 – 3 . POR, which is one of three known light-dependent enzymes 4 , 5 , catalyses reduction of the photosensitizer and substrate protochlorophyllide to form the pigment chlorophyllide. Despite its biological importance, the structural basis for POR photocatalysis has remained unknown. Here we report crystal structures of cyanobacterial PORs from Thermosynechococcus elongatus and Synechocystis sp. in their free forms, and in complex with the nicotinamide coenzyme. Our structural models and simulations of the ternary protochlorophyllide–NADPH–POR complex identify multiple interactions in the POR active site that are important for protochlorophyllide binding, photosensitization and photochemical conversion to chlorophyllide. We demonstrate the importance of active-site architecture and protochlorophyllide structure in driving POR photochemistry in experiments using POR variants and protochlorophyllide analogues. These studies reveal how the POR active site facilitates light-driven reduction of protochlorophyllide by localized hydride transfer from NADPH and long-range proton transfer along structurally defined proton-transfer pathways. Crystal structures of cyanobacterial protochlorophyllide oxidoreductases reveal the basis of the photocatalytic activities of this enzyme, through the role of its active site in enabling the light-driven reduction of protochlorophyllide.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 631/535 / 631/57 / 82 / Biochemistry / Biosynthesis / Catalysis / Chemical properties / Chlorophyll / Chlorophyll - biosynthesis / Chlorophyll - chemistry / Computer simulation / Crystal structure / Cyanobacteria / Dehydrogenases / Elongated structure / Enzymes / Humanities and Social Sciences / Life on Earth / Light / Methods / Molecular Structure / multidisciplinary / NADP / NADPH / Nicotinamide / Observations / Optical properties / Oxidoreductase / Oxidoreductases Acting on CH-CH Group Donors - chemistry / Oxidoreductases Acting on CH-CH Group Donors - metabolism / Photocatalysis / Photochemicals / Photochemistry / Photosensitization / Photosynthesis / Physiological aspects / Proteins / Protochlorophyllide / Protochlorophyllide - metabolism / Protons / Reduction / Science / Science (multidisciplinary) / Solvents / Structural models / Structure / Structure-Activity Relationship / Substrate Specificity / Substrates / Synechococcus - enzymology / Synechocystis - enzymology