Journal Article

Long-range population dynamics of anatomically defined neocortical networks

Chen, Jerry L, Voigt, Fabian F, Krueppel, Roland, Javadzadeh, Mitra, Helmchen, Fritjof

2016

Overview

The coordination of activity across neocortical areas is essential for mammalian brain function. Understanding this process requires simultaneous functional measurements across the cortex. In order to dissociate direct cortico-cortical interactions from other sources of neuronal correlations, it is furthermore desirable to target cross-areal recordings to neuronal subpopulations that anatomically project between areas. Here, we combined anatomical tracers with a novel multi-area two-photon microscope to perform simultaneous calcium imaging across mouse primary (S1) and secondary (S2) somatosensory whisker cortex during texture discrimination behavior, specifically identifying feedforward and feedback neurons. We find that coordination of S1-S2 activity increases during motor behaviors such as goal-directed whisking and licking. This effect was not specific to identified feedforward and feedback neurons. However, these mutually projecting neurons especially participated in inter-areal coordination when motor behavior was paired with whisker-texture touches, suggesting that direct S1-S2 interactions are sensory-dependent. Our results demonstrate specific functional coordination of anatomically-identified projection neurons across sensory cortices. Behavior and cognition – the process of thought – emerge from computations that occur within vast networks of neurons in the brain. Within these networks, neurons may communicate with their neighbours in the same brain region as well as with distant counterparts in remote brain regions. Neuroscientists have studied these networks by measuring the activity of neurons within a single region or across the brain as a whole. However, it has not been possible to study long-distance communication between pairs of neurons in different brain regions. This has made it difficult to work out exactly what information brain regions exchange. Chen, Voigt et al. now overcome these challenges by developing a new microscope system that allows researchers to measure the activity of individual neurons in different brain regions at the same time. The system works alongside tracing techniques that map the connections between distant neurons. To demonstrate the new tools, Chen, Voigt et al. measured the activity of neurons in two areas of the mouse brain that monitor the whiskers. Mice brush their whiskers against an object to obtain information on its size, shape, texture and location. Two brain regions, called the primary and secondary areas of the whisker cortex, process this information and exchange messages back and forth. However, it was unclear what information these messages contain. Chen, Voigt et al. therefore trained mice to discriminate between coarse and fine sandpapers using their whiskers, and analysed the activity of the neurons that directly connect the two areas of the whisker cortex. The results revealed that although movement and sensory stimulation activated both the primary and secondary areas of the whisker cortex, the direct connections between these regions mainly exchange sensory information. This approach makes it possible to observe brain networks in an unprecedented level of detail. In the future, this technology will be extended to provide a more comprehensive view of how neurons communicate across brain areas. This will increase our understanding of how multiple areas of the brain all work together to produce the activity patterns that give rise to behavior.

• Publisher: eLife Science Publications, Ltd,eLife Sciences Publications Ltd,eLife Sciences Publications, Ltd
• Subject: Animals / calcium / Calcium - metabolism / Calcium imaging / cortex / Exploratory Behavior - physiology / Feedback / Lasers / Male / Mice / Mice, Inbred C57BL / Mice, Transgenic / Microscopy, Fluorescence, Multiphoton / Molecular Imaging / Motor Activity - physiology / Neocortex / Neural circuitry / Neural Pathways - physiology / Neural Pathways - ultrastructure / Neuroimaging / Neurons / Neurons - physiology / Neurons - ultrastructure / Neuroscience / Olfactory Perception - physiology / Physiological aspects / Population / Sensory neurons / Somatosensory cortex / Somatosensory Cortex - physiology / Somatosensory Cortex - ultrastructure / Synaptic Transmission / Tracers / two-photon / Vibrissae / whisker