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Global transcriptomic response of Escherichia coli to p-coumaric acid
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Global transcriptomic response of Escherichia coli to p-coumaric acid
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Journal Article
Global transcriptomic response of Escherichia coli to p-coumaric acid
2022
Overview
The aromatic compound
p
-coumaric acid (p-CA) is a secondary metabolite produced by plants. This aromatic acid and derived compounds have positive effects on human health, so there is interest in producing them in biotechnological processes with recombinant
Escherichia coli
strains. To determine the physiologic response of
E. coli
W3110 to p-CA, dynamic expression analysis of selected genes fused to a fluorescent protein reporter as well as RNA-seq and RT-qPCR were performed. The observed transcriptional profile revealed the induction of genes involved in functions related to p-CA active export, synthesis of cell wall and membrane components, synthesis of amino acids, detoxification of formaldehyde, phosphate limitation, acid stress, protein folding and degradation. Downregulation of genes encoding proteins involved in energy production, carbohydrate import and metabolism, as well as several outer and plasma membrane proteins was detected. This response is indicative of cell envelope damage causing the leakage of intracellular components including amino acids and phosphate-containing compounds. The cellular functions responding to p-CA that were identified in this study will help in defining targets for production strains improvement.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Carbon
/ Chemistry and Materials Science
/ E coli
/ Enzymes
/ Genes
/ Lignin
/ Microbial Genetics and Genomics
/ Plasmids
/ Proteins
/ Testing
